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Glycemic, insulinemic and incretin responses after oral trehalose ingestion in healthy subjects
Nutrition Journalvolume 16, Article number: 9 (2017)
Trehalose is hydrolyzed by a specific intestinal brush-border disaccharidase (trehalase) into two glucose molecules. In animal studies, trehalose has been shown to prevent adipocyte hypertrophy and mitigate insulin resistance in mice fed a high-fat diet. Recently, we found that trehalose improved glucose tolerance in human subjects. However, the underlying metabolic responses after trehalose ingestion in humans are not well understood. Therefore, we examined the glycemic, insulinemic and incretin responses after trehalose ingestion in healthy Japanese volunteers.
In a crossover study, 20 fasted healthy volunteers consumed 25 g trehalose or glucose in 100 mL water. Blood samples were taken frequently over the following 3 h, and blood glucose, insulin, active gastric inhibitory polypeptide (GIP) and active glucagon-like peptide-1 (GLP-1) levels were measured.
Trehalose ingestion did not evoke rapid increases in blood glucose levels, and had a lower stimulatory potency of insulin and active GIP secretion compared with glucose ingestion. Conversely, active GLP-1 showed higher levels from 45 to 180 min after trehalose ingestion as compared with glucose ingestion. Specifically, active GIP secretion, which induces fat accumulation, was markedly lower after trehalose ingestion.
Our findings indicate that trehalose may be a useful saccharide for good health because of properties that do not stimulate rapid increases in blood glucose and excessive secretion of insulin and GIP promoting fat accumulation.
Trehalose is a non-reducing disaccharide that consists of two glucose units linked by a α, α-1,1-glucosidic bond. Trehalose is widespread in nature such as plants, fungi and insects, and helps to preserve the life of plants and animals [1, 2]. Although trehalose was previously consumed from natural sources, it has been manufactured from starch through a proprietary enzymatic process since 1995. As a result, trehalose is widely used in a variety of foods to protect against dryness, freezing and osmotic pressure stresses.
In previous studies, we showed that trehalose intake prevented adipocyte hypertrophy and mitigated insulin resistance in mice fed a high-fat diet [3, 4]. In addition, it has been reported that trehalose has various biological effects such as suppression of bone resorption  and inflammatory response , induction of autophagy [7, 8] and alleviation of Huntington’s disease . Although these effects were found in vitro or in vivo studies, there was little information available about the physiological functions of trehalose in humans.
Oku et al. have reported that trehalose is a carbohydrate that does not raise blood glucose levels quickly and induces lower insulin secretion compared with glucose in female students (age 21.8 ± 5.4) ; however, data from a broad age-range of people, including men, remain unknown. Furthermore, the incretin response related to glucose metabolism following trehalose intake has not been examined in human subjects. Incretins are gut hormones that are secreted from enteroendocrine cells into the blood after eating and promote insulin secretion . Gastric inhibitory polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) are main incretins in humans. In recent years, incretin receptor has been detected in many organs, and its various physiological extrapancreatic effects have received a lot of attention . For example, it is known that GIP promotes bone formation  and fat accumulation in adipose tissue . This incretin also has been reported to suppress gastric acid secretion in the gastrointestinal tract . In addition, GLP-1 promotes cardioprotection in the heart  and delay gastric emptying in the gastrointestinal tract . In this study, we examined glycemic, insulinemic and incretin responses after oral trehalose ingestion in healthy human subjects.
Twenty-four healthy Japanese subjects (13 women and 11 men; mean age = 37.8 ± 1.6 years, BMI = 20.7 ± 0.3 kg/m2) were recruited. All subjects were informed about the purpose, methods, and possible risks of the study before giving their consent to participate.
This study was approved by the Ethics Committee of Hayashibara Co. Ltd. (approval No. 152) and the Nishi Clinic Ethics Committee (approval No. R1301) (Osaka, Japan). And this study was carried out in Oneness Support Co., Ltd., and the Miura Clinic (Osaka, Japan). All studies were conducted in accordance with the Declaration of Helsinki (adopted in 1964 and revised in 2008) and Japan’s Ethical Guidelines for Epidemiology Research (adopted in 2002 and revised in 2004).
TREHA™ (Hayashibara Co. Ltd., Okayama, Japan) was used as the source of trehalose. It contains more than 98.0% of trehalose dihydrate. Glucose“Yoshida” (Yoshida Pharmaceutical Inc., Tokyo, Japan) consists of 100% glucose anhydrous.
Subjects were studied following an overnight fast in the morning on two occasions with an interval of at least 1 week. Trehalose and glucose were ingested during two different trials using a cross-over design.
Although trehalose is hydrolyzed in the small intestine, it was reported that some people caused high osmotic diarrhea because trehalose was not digested completely when large enough amounts were ingested at the same time . In this study, therefore, we decided the quantity of load substance as 25 g, not 50 g generally used for Glycemic Index determination.
Twenty-five grams of trehalose (i.e., 28 g of TREHA™) or glucose were dissolved in 100 mL water and ingested in a gross quantity within 2 min. Venous blood samples were taken before and 15, 30, 45, 60, 90, 120 and 180 min after ingestion. All blood samples were divided at each time point for use in measurements of plasma glucose (Ultraviolet absorption spectrophotometer), serum insulin (Chemiluminescent enzyme immunoassay), plasma active GIP and active GLP-1 (Solid-phase extraction-enzyme-linked immunosorbent assay) by SRL, Inc. (Nara, Japan).
Time to maximum plasma or serum concentrations (Tmax) and the maximum plasma or serum concentrations (Cmax) were directly obtained from the plasma or serum concentration time-course data.
The area under the curve for blood glucose, insulin, active GIP or active GLP-1 levels was calculated as the incremental area under the response curve (IAUC), ignoring the area beneath the fasting concentration.
Each outcome measure is expressed as the mean ± the standard error (SE). Paired Student’s t-tests were applied for the analysis of Tmax, Cmax and IAUC between trehalose and glucose ingestion. For plasma or serum time curves, two–way repeated measures ANOVA (MANOVA) analyze with ingestion, time, and their interaction was used to identify differences between treatments over time. When interaction or ingestion effects were observed, Wilcoxon post hoc analysis was performed to locate these differences. A value of p < 0.05 was considered to be statistically significant. All calculations were performed with JMP 9 (SPS Institute Inc.).
Analytical subjects of this study
Among 24 participants in this study, one subject was shown to be anemic at blood sampling, two subjects experienced borborygmus and diarrhea after receiving the investigational test substance, and one subject was suspected to have insulin resistance because of high fasting blood insulin levels. Diarrhea symptoms emerged at 50 and 90 min after ingestion of trehalose in the two subjects, with the diarrhea present for several hours. A medical review suggested the presence of osmotic diarrhea due to incompleted digestion in the small intestine and hence the subjects were withdrawn. Therefore, we surveyed the data of 20 subjects (ten women and ten men; mean age = 39.0 ± 1.6 years: range = 23–48, BMI = 20.8 ± 0.3 kg/m2: range = 18.3–23.5) as the final analytical results in this study.
Change of blood glucose and insulin levels
Postprandial venous blood glucose and insulin levels are presented in Fig. 1. Trehalose ingestion significantly lowered blood glucose and insulin peaks compared with glucose ingestion. Blood glucose and insulin levels observed in trehalose loading were significantly lower than those in glucose loading at 15, 30, 45 and 60 min after ingestion. Conversely, blood glucose values at 120 and 180 min, and insulin values at 180 min after trehalose ingestion were significantly higher than those after glucose ingestion.
Table 1 showed the metabolic responses after ingestion of trehalose or glucose. Tmax of blood glucose and insulin did not differ between trehalose and glucose loading. Cmax and IAUC(0-2 h) of blood glucose and insulin in trehalose loading were significantly lower than those in glucose loading.
Change of blood incretin levels
Cmax of active GIP in trehalose loading was significantly lower than that in glucose loading (Table 1). Furthermore, active GIP levels observed in trehalose loading were lower than those in glucose loading at 15, 30, 45, 60 and 90 min after ingestion (Fig. 2).
Cmax of active GLP-1 was not significantly different between trehalose and glucose loading (Table 1). Moreover, active GLP-1 levels observed at 45, 60, 90, 120 and 180 min after trehalose ingestion were significantly higher than those after glucose ingestion (Fig. 2).
As a result of experiments in healthy human subjects, both blood glucose and insulin levels were found to be significantly lower following trehalose loading compared with glucose loading. Furthermore, active GIP levels were markedly lower following trehalose loading compared with glucose loading. Sex differences were not found in these responses (data not presented).
Diarrhea symptom was observed in the two subjects when 25 g of trehalose were dissolved in 100 mL water and administered in this study. Whereas sixty German subjects were given a 50 g dose of trehalose, on the other hand, diarrhea and malabsorption were not observed in any of the subjects as reported by Bolte et al. . In their study, 400 mL of water was used to administer 50 g of trehalose. In case of Oku et al. , furthermore, several dosages of trehalose dissolved in 100–150 mL of tap water were given and no subjects had diarrhea when administered at least 30 g of trehalose. Together with these results, it is possible that diarrhea symptom is caused by the concentration of trehalose solution when given to subject but not total quantity.
Individual blood glucose IAUC after trehalose ingestion was lower than after glucose ingestion. Although a blood glucose IAUC ratio of trehalose loading to glucose loading was individually different, the mean of these values for all subjects was 38% in the present study. This value is equivalent to the glycemic index, which was introduced to classify carbohydrate foods according to the degree of postprandial glycemia. In general, low glycemic index foods improve blood glucose control, the blood lipid profile and fibrinolytic activity in diabetic subjects [19, 20]. Furthermore, postprandial spikes in blood glucose can cause damage to fragile blood vessels in the heart, brain, kidneys, eyes and feet [21–23]. Trehalose might help prevent these risks because it did not rapidly raise blood glucose levels after ingestion, but requires evealuation for potential usefulness as a sweetener for diabetic patients.
Moreover, the mean individual blood insulin IAUC ratio of trehalose loading to glucose loading was 36%. Hyperinsulinemia characterizes impaired glucose tolerance and obesity, and reflects peripheral insulin resistance . Therefore, food materials that do not cause a rapid rise in blood glucose levels and excessive insulin secretion have been expected to prevent onset and progression of lifestyle-related diseases.
Interestingly, active GIP secretion following trehalose loading was conspicuously lower than that of glucose loading. The mean individual blood GIP IAUC ratio of trehalose loading to glucose loading was only 14%. GIP is secreted from K cells, which exist in the upper small intestine, by the stimulation of nutrients such as carbohydrates and lipids . It is thought that ingested trehalose provides little stimulation to K cells during the process of digestion and absorption in the gastrointestinal tract. The lower active GIP concentration found in this study might be one of the mechanisms behind lower insulin release following trehalose ingestion. Recently, a relationship between GIP and obesity has been clarified, and it has been understood that GIP has a physiological role in the nutrient uptake into adipose tissues, thereby linking over nutrition to obesity [14, 25]. Furthermore, it has been reported that GIP receptor-deficient mice fed a high-fat diet were protected from obesity . Restraining GIP signals may lead to the reduction of disease risk such as obesity. In previous reports, we showed that the intake of trehalose suppressed adipocyte hypertrophy and mitigated insulin resistance in mice fed a high-fat diet [3, 4]. Additionally, we confirmed that the consumption of trehalose with a meal three times daily improved glucose tolerance and mitigated the progression of insulin resistance in humans . Low secretion characteristics of GIP may contribute to the suppression of adipocyte hypertrophy and insulin resistance.
In contrast, it is known that GLP-1 protects pancreatic β-cells and suppresses appetite. In this study, although the active GLP-1 levels following trehalose loading were higher than those following glucose loading from 45 to 180 min after ingestion, the levels were very low probably because of rapid disintegration from the enzyme dipeptidyl peptidase-4 (DPP-4). Therefore, we could not argue that trehalose loading led to higher levels of active GLP-1 secretion than glucose loading in this study. A further study is planned in our laboratory.
Recently, DeBosch et al. [8, 27] proposed a model that trehalose inhibits multiple glucose transporter (GLUT) family members through docking in the inward open conformation of glucose transporters. Therefore, trehalose may prevent excessive glucose absorption in the small intestine by inhibiting glucose transporter function. Furthermore van Can et al.  reported the implications for postprandial trehalose ingestion reduced glycemic responses in impaired glucose-tolerant subjects. Therefore a consuming trehalose with daily meals may contribute to the maintenance of good health through the prevention of hyperglycemia. Also trehalose may possibly contribute with useful saccharide in prediabetic and type 2 diabetic patients. These possibilities must be resolved in the future.
In conclusion, we have shown that it is hard for trehalose to give rise to hyperglycemia and hypersecretions of insulin and GIP following ingestion. These results indicate that trehalose may be a useful saccharide for good health because of properties that do not stimulate rapid increases in blood glucose and excessive secretion of insulin and GIP promoting fat accumulation.
Gastric inhibitory polypeptide
Time to maximum concentrations
Incremental area under the curve
Body mass index
Elbein AD, Pan YT, Pastuszak I, Carroll D. New insights on trehalose: a multifunctional molecule. Glycobiology. 2003;13:17R–27R.
Colaço C, Sen S, Thangavelu M, Pinder S, Rose B. Extraordinary stability of enzymes dried in trehalose: simplified molecular biology. Biotechnology (N Y). 1992;10:1007–11.
Arai C, Arai N, Mizote A, Kohno K, Iwaki K, Hanaya T, Arai S, Ushio S, Fukuda S. Trehalose prevents adipocyte hypertrophy and mitigates insulin resistance. Nutr Res. 2010;30:840–8.
Arai C, Miyake M, Matsumoto Y, Mizote A, Yoshizane C, Hanaya Y, Koide K, Yamada M, Hanaya T, Arai S, Fukuda S. Trehalose prevents adipocyte hypertrophy and mitigates insulin resistance in mice with established obesity. J Nutr Sci Vitaminol. 2013;59:393–401.
Nishizaki Y, Yoshizane C, Toshimori Y, Arai N, Akamatsu S, Hanaya T, Arai S, Ikeda M, Kurimoto M. Disaccharide-trehalose inhibits bone resorption in ovariectomized mice. Nutr Res. 2000;20:653–64.
Arai C, Kohguchi M, Akamatsu S, Arai N, Yoshizane C, Hasegawa N, Hanaya T, Arai S, Ikeda M, Kurimoto M. Trehalose suppresses lipopolysaccharide-induced osteoclastogenesis bone marrow in mice. Nutr Res. 2001;21:993–99.
Belzile JP, Sabalza M, Craig M, Clark E, Morello CS, Spector DH. Trehalose, an mTOR-independent inducer of autophagy, inhibits human cytomegalovirus infection in multiple cell types. J Virol. 2015;90:1259–77.
DeBosch BJ, Heitmeier MR, Mayer AL, Higgin CB, Crowly JR, Kraft TE, Chi M, Newberry EP, Chen Z, Finck BN, Davidson NO, Yarasheski KE, Hruz PW, Moley KH. Trehalose inhibits solute carrier 2A (SLC2A) proteins to induce autophagy and prevent hepatic steatosis. Sci Signal. 2016;9:ra21. doi:10.1126/scisignal.aac5472.
Tanaka M, Machida Y, Niu S, Ikeda T, Jana NR, Doi H, Kurosawa M, Nekooki M, Nukina N. Trehalose alleviates polyglutamine-mediated pathology in a mouse model of Huntington disease. Nat Med. 2004;10:148–54.
Oku T, Nakamura S. Estimation of intestinal trehalase activity from a laxative threshold of trehalose and lactulose on healthy female subjects. Eur J Clin Nutr. 2000;54:783–88.
Kim W, Egan JM. The Role of incretins in glucose homeostasis and diabetes treatment. Pharmacol Rev. 2008;60:470–512.
Seino Y, Fukushima M, Yabe D. GIP and GLP-1, the two incretin hormones: similarities and differences. J Diabetes Investig. 2010;1:8–23.
Nissen A, Christensen M, Knop FK, Vilsbøll T, Holst JJ, Hartmann B. Glucose-dependent insulinotropic polypeptide inhibits bone resorption in humans. J Clin Endocrinol Metab. 2014;99:E2325–9.
Getty-Kaushik L, Song DH, Boylan M, Corkey BE, Wolfe MM. Glucose-dependent insulinotropic polypeptide modulates adipocyte lipolysis and reesterification. Obesity. 2006;14:1124–31.
Pederson RA, Brown JC. Inhibition of histamine-, pentagastrin-, and insulin-stimulated canine gastric secretion by pure “gastric inhibitory polypeptide”. Gastroenterology. 1972;62:393–400.
Ravassa S, Zudaire A, Díez J. GLP-1 and cardioprotection: from bench to bedside. Cardiovasc Res. 2012;94:316–23.
I˙meryüz N, Yeğen BÇ, Bozkurt A, Coşkun T, Villanueva-Peñacarrillo ML, Ulusoy NB. Glucagon-like peptide-1 inhibits gastric emptying via vagal afferent-mediated central mechanisms. Am J Physiol Gastrointest Liver Physiol. 1997;273:G920–7.
Bolte JP, Schönhage F, Förster E, Knolle J, Büschenfelde KH M z. Zur diagnostischen bedeutung der trehalose-belastung bei malassimilationssyndromen. Dtsch Med Wochenschr. 1973;98:1358–62.
Jenkins DJ, Kendall CW, Augustin LS, Franceschi S, Hamidi M, Marchie A, Jenkins AL, Axelsen M. Glycemic index: overview of implications in health and disease. Am J Clin Nutr. 2002;76:266S–73S.
Järvi AE, Karlström BE, Granfeldt YE, Björck IE, Asp NG, Vessby BO. Improved glycemic control and lipid profile and normalized fibrinolytic activity on a low-glycemic index diet in type 2 diabetic patients. Diabetes Care. 1999;22:10–8.
Ceriello A. Impaired glucose tolerance and cardiovascular disease: the possible role of post-prandial hyperglycemia. Am Heart J. 2004;147:803–7.
Prasad S, Sajja RK, Naik P, Cucullo L. Diabetes mellitus and blood–brain barrier dysfunction: An overview. J Pharmacovigilance. 2014;2:125.
Hippisley-Cox J, Coupland C. Diabetes treatments and risk of amputation, blindness, severe kidney failure, hyperglycaemia, and hypoglycaemia: open cohort study in primary care. Br Med J. 2016;352:i1450. doi:10.1136/bmj.i1450.
Modan M, Halkin H, Almog S, Lusky A, Eshkol A, Shefi M, Shitrit A, Fuchs Z. Hyperinsulinemia. A link between hypertension obesity and glucose intolerance. J Clin Investig. 1985;75:809–17.
Nasteska D, Harada N, Suzuki K, Yamane S, Hamasaki A, Joo E, Iwasaki K, Shibue K, Harada T, Inagaki N. Chronic reduction of GIP secretion alleviates obesity and insulin resistance under high-fat diet conditions. Diabetes. 2014;63:2332–43.
Mizote A, Yamada M, Yoshizane C, Arai N, Maruta K, Arai S, Endo S, Ogawa R, Mitsuzumi H, Ariyasu T, Fukuda S. Daily intake of trehalose is effective in the prevention of lifestyle-related diseases in individuals with risk factors for metabolic syndrome. J Nutr Sci Vitaminol. 2016;62:380–7.
Mayer AL, Higgins CB, Heitmeier MR, Kraft TE, Qian X, Crowley JR, Hyrc KL, Beatty WL, Yarasheski KE, Hruz PW, DeBosch BJ. SLC2A8 (GLUT8) is a mammalian trehalose transporter required for trehalose-induced autophagy. Scientific Reports. 2016;6:38586. doi:10.1038/srep38586.
Van Can JG, Ijzerman TH, van Loon LJ, Brouns F, Blaak EE. Reduced glycaemic and insulinaemic responses following trehalose and isomaltulose ingestion: implications for postprandial substrate use in impaired glucose-tolerant subjects. Br J Nutr. 2012;108:1210–7.
We are grateful to the study participants for their cooperation and participation. We thank Mr. Takashi Kamiya and Dr. Chikako Arai from HAYASHIBARA CO. LTD. for their comments on the English in the manuscript.
Availability of data and material
MY, AM, NA, CY, SA, KM, HM and TA designed the study. AM and NA oversaw data collection; AM, CY, NA, SA and KM performed statistical analyses, wrote the manuscript, and had primary responsibility for the final content. HM, TA, SU and SF were involved in revision of the manuscript and the final version of the manuscript. All authors read and approved the final manuscript.
All authors are employees of HAYASHIBARA CO. LTD., the study sponsor. The authors declare that they have no other conflict of interest.
Consent for publication
Ethics approval and consent to participate
Ethical approval (approval No. R1301) was obtained from the Nishi Clinic Ethics Committee (Osaka, Japan). All subjects were informed about the purpose, methods, and possible risks of the study before giving their consent to participate.