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A whey protein supplement decreases post-prandial glycemia
- Brent L Petersen†2,
- Loren S Ward†2Email author,
- Eric D Bastian†2,
- Alexandra L Jenkins†1,
- Janice Campbell†1 and
- Vladimir Vuksan†1
© Petersen et al; licensee BioMed Central Ltd. 2009
Received: 11 May 2009
Accepted: 16 October 2009
Published: 16 October 2009
Incidence of diabetes, obesity and insulin resistance are associated with high glycemic load diets. Identifying food components that decrease post-prandial glycemia may be beneficial for developing low glycemic foods and supplements. This study explores the glycemic impact of adding escalating doses of a glycemic index lowering peptide fraction (GILP) from whey to a glucose drink.
Ten healthy subjects (3M, 7F, 44.4 ± 9.3 years, BMI 33.6 ± 4.8 kg/m2) participated in an acute randomised controlled study. Zero, 5, 10 and 20 g of protein from GILP were added to a 50 g glucose drink. The control (0 g of GILP) meal was repeated 2 times. Capillary blood samples were taken fasting (0 min) and at 15, 30, 45, 60, 90 and 120 minutes after the start of the meal and analyzed for blood glucose concentration.
Increasing doses of GILP decreased the incremental areas under the curve in a dose dependant manner (Pearson's r = 0.48, p = 0.002). The incremental areas (iAUC) under the glucose curve for the 0, 5, 10, and 20 g of protein from GILP were 231 ± 23, 212 ± 23, 196 ± 23, and 138 ± 13 mmol.min/L respectively. The iAUC of the 20 g GILP was significantly different from control, 5 g GILP and 10 g GILP (p < 0.001). Average reduction in the glucose iAUC was 4.6 ± 1.4 mmol.min/L per gram of ingested GILP.
Addition of GILP to a oral glucose bolus reduces blood glucose iAUC in a dose dependent manner and averages 4.6 ± 1.4 mmol.min/L per gram of GILP. These data are consistent with previous research on the effect of protein on the glycemic response of a meal.
The rate of glucose absorption and the duration of elevated blood glucose levels induce many hormonal and metabolic changes that may affect health or disease parameters. Low glycemic index (GI) diets may help in weight maintenance and weight loss  in addition to being protective against chronic disease such as diabetes , heart disease [3, 4] and certain cancers [5, 6]. Interest in identifying low GI foods and the food factors responsible for the low GI of foods has therefore increased. Several food factors have been identified that influence in vivo absorption and therefore potentially the GI of a food or meal. Some of these factors include: the food matrix, cell wall and starch structure (i.e. ripening), amylose to amylopectin ratio, protein-starch interaction, processing and dietary fibre .
More recently, interest has focused on the metabolic responses to dietary proteins. Proteins vary in their ability to decrease post-prandial glycemia. Comparing the insulinotropic characteristics of milk, gluten, cod, cheese and whey demonstrated that both milk and whey have the greatest impact on glucose metabolism by increasing both insulin secretion and glucose-dependent insulinotropic polypeptide (GIP) .
Milk protein, in particular, appears to stimulate an increase in postprandial insulin response with a corresponding reduction in postprandial blood glucose levels [9, 10]. Studies exploring the insulinotropic effect of the dairy protein have found that the whey fraction seems to contain the predominating insulinotropic secretagogue [8, 11, 12].
Different sources of protein may be digested at different rates; with whey being one of the most rapidly digested resulting in high postprandial concentrations of amino acids . Individual amino acids may act as potent insulin secretagogues [13, 14] and in particular, leucine, isoleucine, valine, lysine and threonine have been proposed as the most likely amino acids responsible for the increase seen in insulin concentrations . It is not known if addition of whey protein will consistently lower postprandial blood glucose responses and whether this lowering happens in a dose responsive manner.
This study was therefore conducted to measure the impact of a glycemic index lowering peptide ingredient given in escalating doses to a glucose drink on post prandial glycemia and to calculate the relative glycemic lowering ability of this protein fraction.
Ten healthy subjects participated in the study consisting of 3 males and 7 females with an average age of (Mean ± SD) 44.4 ± 9.3 years. The body mass index of the subjects ranged from 26.7 to 41.1 kg/m2, the average being 33.6 ± 4.8 kg/m2. Subjects were recruited through the Glycemic Index Laboratories clinic volunteer roster. The study was approved by the Western Institutional Review Board, Washington. Informed written consent was obtained from all subjects prior to the start of the study. Subjects received a financial reward for their participation. All 6 treatments were completed on separate days with at least one day between tests.
Composition of the different meals.
5 g GILP
10 g GILP
20 g GILP
The protein supplement (Glanbia, Twin Falls, ID USA) consisted of a blend of whey peptides and intact whey protein containing a high concentration of branch chain amino acids. Doses were calculated to contain 5, 10 and 20 g of protein.
On each test day, subjects came to Glycemic Index Laboratories, Inc. in the morning after a 10-14 h overnight fast. After being weighed and having a fasting blood sample obtained by finger-prick, the subject then consumed a test meal within 10 minutes, and further blood samples were obtained at 15, 30, 45, 60, 90 and 120 minutes after the start of the test meal.
A beverage of choice was served to each subject with each test meal, comprising of one or two cups of water, tea or coffee with or without milk. The same beverage was served to the subject at each of the subsequent test meals.
The incremental area under the plasma glucose response curve (iAUC) for each of the test meals for each subject was calculated using the trapezoid rule and not using the area under the baseline . The mean of the two glucose meals (control) was used in statistical analysis and the intra-individual coefficient of variation and intraclass coefficient of correlation of the two OGTTs were calculated. Differences between the meals were assessed using repeated-measures analysis of variance (ANOVA) and adjusted for multiple pair-wise comparisons with the Tukey-Kramer procedure. Relationship between glucose iAUC levels and GILP dose was calculated using the Pearson's correlation and linear regression to estimate the reduction in iAUC per gram of GILP. All data are expressed as Mean ± SEM.
The means of the iAUC of the two glucose controls were 237 ± 25 and 225 ± 24 mmol/L·min. The intra-individual coefficient of variation and intra-class coefficient of correlation of the repeated control glucose test meals were 28.3% and 17.9% respectively.
Glucose iAUC levels correlated inversely with GILP doses, indicating that higher doses of GILP resulted in lower blood glucose levels (Pearson's r = -0.48, p = 0.002). Linear regression analysis showed significant dose-response decrease in blood glucose iAUC with increasing doses of GILP (slope = -4.6 ± 1.4; t = -3.4; p = 0.002; Y = 235-4.6X; r 2 = 0.23).
This study demonstrated that addition of this unique glycemic index lowering peptide whey-based protein reduces postprandial glycemia in a dose-dependent manner when added to a 50 g glucose drink. The exact mechanism is not known but it has been postulated that the blood glucose lowering is a result of an increase of insulin secretion [11, 12]. In studies where protein is combined with carbohydrate, an enhancement of the insulin response is observed [11, 16].
The differential effect of different amino acids on postprandial insulin levels is well known, it is therefore likely that protein mixes with different amino acid profiles will elicit different postprandial insulin responses. Whey protein is particularly high in branch-chain amino acids, in particular leucine . These amino acids are insulinogenic, meaning that they have a higher capacity to increase an insulin response , which may account for the results seen in this study. Another possibility is the effect of whey protein on incretin hormones released in the gut [19, 20]. Incretin hormones such as Glucagon-like-peptide-1 (GLP-1) and Glucose-dependent insulinotropic peptide (GIP) are released from the gut after food consumption and are involved in many digestive roles including glycemic control.
Glucagon-like peptide-1 (GLP-1) is a multi-functioning hormone which is released from the gut in response to a meal. Exogenous GLP-1 stimulates insulin secretion and individuals with diabetics have lower levels of GLP-1 secretion .
Dipeptidyl peptidase IV (DPP-IV) is a peptidase which hydrolyzes incretin hormones like GLP-1. Inhibiting DPP-IV in rats with an inhibitor has been shown to suppress blood glucose elevation by possibly protecting GLP-1 . One important role whey protein may play is in the physiology of incretin hormones. Whey protein has been shown in mice to raise GLP-1 and inhibit DPP-IV resulting in an increased and prolonged insulin response [19, 20]. Measurement of these hormones after ingestion of meals containing GILP may help elucidate the mechanism of action of the glucose reduction seen with this whey peptide fraction.
In conclusion, this unique glycemic index lowering peptide whey based protein significantly lowered the glycemic response elicited by 50 g of glucose in a dose dependent manner. The reduction in blood glucose iAUC per gram of protein powder was calculated to be 4.6 ± 1.4 mmol.min/L. Addition of GILP to carbohydrate food products may be an effective way of lowering the glycemic impact of these foods.
- Ebbeling CB, Leidig MM, Sinclair KB, Hangen JP, Ludwig DS: A reduced-glycemic load diet in the treatment of adolescent obesity. Arch Pediatr Adolesc Med. 2003, 157: 773-779. 10.1001/archpedi.157.8.773.View ArticlePubMedGoogle Scholar
- Salmerón J, Ascherio A, Rimm EB, Colditz GA, Spiegelman D, Jenkins DJ, Stampfer MJ, Wing AL, Willett WC: Dietary fiber, glycemic load, and risk of NIDDM in men. Diabetes care. 1997, 20 (4): 545-550. 10.2337/diacare.20.4.545.View ArticlePubMedGoogle Scholar
- Van Dam RM, Visscher AEJ, Feskens EJM, Verhoef P, Kromhout D: Dietary glycemic index in relation to metabolic risk factors and incidence of coronary heart disease: the Zutphen Elderly Study. Euro Journal Clin Nutr. 2000, 54: 726-731. 10.1038/sj.ejcn.1601086.View ArticleGoogle Scholar
- Liu S, Manson JE, Buring JE, Stampfer MJ, Willett WC, Ridker PM: Relation between a diet with a high glycemic load and plasma concentrations of high-sensitivity C-reactive protein in middle-aged women. Am J Clin Nutr. 2002, 75: 492-498.PubMedGoogle Scholar
- Augustin LSA, Polesel J, Bosetti C, Kendall CWC, La Vecchia C, Parpinel M, Conti E, Montella M, Franceschi S, Jenkins DJA, Dal Maso L: Dietary glycemic index, glycemic load and ovarian cancer risk: a case-control study in Italy. Ann Oncol. 2003, 14: 78-84. 10.1093/annonc/dkg022.View ArticlePubMedGoogle Scholar
- Franceschi S, Dal Maso L, Augustin L, Negri E, Parpinel M, Boyle P, Jenkins DJA, La Vecchia C: Dietary glycemic load and colorectal cancer risk. Ann Oncol. 2001, 12: 173-178. 10.1023/A:1008304128577.View ArticlePubMedGoogle Scholar
- Brand JC, Nicholson PL, Thorburn AW, Truswell AS: Food processing and the glycemic index. Am J Clin Nutr. 1985, 42: 1192-1196.PubMedGoogle Scholar
- Nilsson M, Stenberg M, Frid AH, Holst JJ, Bjorck IM: Glycemia and insulinemia in healthy subjects after lactose-equivalent meals of milk and other food proteins: the role of plasma amino acids and incretins. Am J Clin Nutr. 2004, 80 (5): 1246-1253.PubMedGoogle Scholar
- Ostman EM, Elmstahl HGML, Bjorck IME: Inconsistency between glycemic and insulinemic responses to regular and fermented milk products. Am J Clin Nutr. 2001, 74: 96-100.PubMedGoogle Scholar
- Liljeberg Elmstahl H, Bjorck I: Milk as a supplement to mixed meals may elevate postprandial insulinaemia. Euro Journal Clin Nutr. 2001, 55: 994-999. 10.1038/sj.ejcn.1601259.View ArticleGoogle Scholar
- Zawadzki KM, Yaspelkis BB, Ivy JL: Carbohydrate-protein complex increases the rate of muscle glycogen storage after exercise. J Appl Physiol (Bethesda, Md: 1985). 1992, 72 (5): 1854-1859.Google Scholar
- Van Loon LJC, Kruijshoop M, Verhagen H, Saris WHM, Wagenmakers AJM: Ingestion of protein hydrolysate and amino acid-carbohydrate mixtures increases postexercise plasma insulin responses in men. J Nutr. 2000, 130: 2508-2513.PubMedGoogle Scholar
- Schmid R, Schusdziarra V, Schulte-Frohlinde E, Maier V, Classen M: Effect of CCK on insulin, glucagon, and pancreatic polypeptide levels in humans. Pancreas. 1989, 4 (6): 653-661. 10.1097/00006676-198912000-00001.View ArticlePubMedGoogle Scholar
- Krebs M, Brehm A, Krssak M, Anderwald C, Bernroider E, Nowotny P, Roth E, Chandramouli V, Landau BR, Waldhäusl W, Roden M: Direct and indirect effects of amino acids on hepatic glucose metabolism in humans. Diabetologia. 2003, 46 (7): 917-925. 10.1007/s00125-003-1129-1.View ArticlePubMedGoogle Scholar
- Wolever TMS, Jenkins DJA, Jenkins AL, Josse RG: The glycemic index: methodology and clinical implications. Am J Clin Nutr. 1991, 54: 846-854.PubMedGoogle Scholar
- Schenk S, Davidson CJ, Zderic TW, Byerley LO, Coyle EF: Different glycemic indexes of breakfast cereals are not due to glucose entry into blood but to glucose removal by tissue. Am J Clin Nutr. 2003, 78 (4): 742-748.PubMedGoogle Scholar
- Layman DK: The role of leucine in weight loss diets and glucose homeostasis. J Nutr. 2003, 133 (1): 261S-267S.PubMedGoogle Scholar
- Rocha DM, Faloona GR, Unger RH: Glucagon-Stimulating Activity of 20 Amino Acids in Dogs. J Clin Invest. 2004, 51: 2346-2351. 10.1172/JCI107046.View ArticleGoogle Scholar
- Gunnarsson PT, Winzell MS, Deacon CF, Larsen MO, Jelic K, Carr RD, Ahren B: Glucose-Induced Incretin Hormone Release and Inactivation Are Differently Modulated by Oral Fat and Protein in Mice. Endocrinology. 2006, 147 (7): 3173-3180. 10.1210/en.2005-1442.View ArticlePubMedGoogle Scholar
- Drucker DJ: Enhancing the Action of Incretin Hormones: A New Whey Forward?. Endocrinology. 2006, 147 (7): 3171-3172. 10.1210/en.2006-0494.View ArticlePubMedGoogle Scholar
- Zander M, Madsbad S, Madsen JL, Holst JJ: Effect of 6-week course of glucagon-like peptide 1 on glycaemic control, insulin sensitivity, and B-cell function in type 2 diabetes: a parallel-group study. Lancet. 2002, 359: 824-830. 10.1016/S0140-6736(02)07952-7.View ArticlePubMedGoogle Scholar
- Takasaki K, Nakajima T, Ueno K, Nomoto Y, Higo K: Effects of combination treatment with dipeptidyl peptidase IV inhibitor and sulfonylurea on glucose levels in rats. J Pharmacol Sci. 2004, 95 (2): 291-293. 10.1254/jphs.SC0040043.View ArticlePubMedGoogle Scholar
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