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Serum lipid responses to psyllium fiber: differences between pre- and post-menopausal, hypercholesterolemic women
Nutrition Journal volume 7, Article number: 22 (2008)
Cardiovascular disease is the leading cause of death in women and men. Psyllium, a soluble fiber has been known to reduce serum lipids. In this pilot study, we evaluated whether menopausal status would affect the serum lipid responses to psyllium fiber in women.
Eleven post-menopausal and eight pre-menopausal women with serum total cholesterol >200 mg/dL were included in the study. Subjects consumed their habitual diet and 15 g psyllium/d for 6 weeks. Psyllium was incorporated into cookies. Each cookie contained ≈5 g of psyllium fiber. Subjects ate one cookie in each meal.
With psyllium fiber, total cholesterol concentration was significantly lower (≈5.2%, P < 0.05) in post-menopausal women but not in pre-menopausal women (≈1.3%). Also, there was a significant decrease in HDL-cholesterol in post-menopausal women (≈10.2%, P < 0.05). There were no significant changes observed in concentrations of LDL-cholesterol, triglycerides, apolipoprotein A1, and apolipoprotein B in both pre- and post-menopausal women with psyllium.
In this pilot study, post- and pre-menopausal, hypercholesterolemic women responded differently to psyllium fiber supplementation. Post-menopausal women would benefit from addition of psyllium to their diets in reducing the risk for heart diseases. The results of this study should be used with caution because the study was based on a small sample size.
Cardiovascular disease (CVD) is the leading cause of death among women and men. About 13 million Americans have CVD and approximately 450,000 people die each year in the US from CVD . Risk factors for CVD in women include post-menopausal status, age, hypertension, smoking, and diabetes . The onset of menopause coincides with elevated serum lipids such as total cholesterol, LDL-cholesterol, and triglycerides and decreased HDL-cholesterol . Elevated serum lipids in post-menopausal women are partly due to the loss of estrogen . Hormone therapy showed a reduction in LDL-cholesterol and an increase in HDL-cholesterol . It also frequently increased triglycerides by increasing the production of triglyceride-rich VLDLs . Additionally, hormone therapy increased the risk for stroke, breast cancer, and cholecystitis . Hormone replacement therapy is not for all post-menopausal women .
Generally, relative to drug therapy, dietary intervention is less expensive and cost effective for primary intervention of heart diseases . Consumption of foods containing dietary fiber, may improve the long-term maintenance of low atherogenic LDL-cholesterol . Psyllium (Plantago ovata) seed husk fiber, a widely used soluble fiber, has been known to reduce serum total cholesterol and LDL-cholesterol [11–13]. Additionally, psyllium fiber supplementation with 10 mg of simvastatin (hypocholesterolemic drug) was as effective as 20 mg of simvastatin alone . This suggests that psyllium fiber is an effective adjuvant hypocholesterolemic agent.
There is a paucity of data on how fiber therapy modulates the risk for CVD in women. Previous studies conducted in women indicated that dietary therapy for lowering serum lipids differs according to menopausal status . In men compared to post-menopausal women, a greater decrease was observed in total cholesterol after 4 months of high fiber diet  suggesting that gender differences exist in hypocholesterolemic effect of dietary fiber. Limited studies were conducted comparing lipid responses to psyllium fiber intervention in pre- and post-menopausal women. Therefore, we tested the hypothesis of whether serum lipid responses to psyllium fiber intake in post-menopausal, hypercholesterolemic women differ from pre-menopausal, hypercholesterolemic women.
A total of 25 hypercholesterolemic women (13 pre-menopausal and 12 post-menopausal) were recruited for the study. Hypercholesterolemia was defined as having serum cholesterol >200 mg/dL. Subjects were recruited from San Francisco and surrounding communities through posting of flyers. Participants lived in their homes during the study. The exclusion criteria were smoking, diabetes, pregnancy, breast feeding, allergic to psyllium fiber, and blood cholesterol ≤200 mg/dL. Individuals with thyroid and gastrointestinal diseases, CVD, chronic alcohol and drug abuse, individuals who were taking lipid-lowering and/or anti-hypertensive medications, and individuals who were on estrogen replacement therapy were excluded from the study. Also, strict vegetarians (vegans) were excluded from the study because their habitual fiber intake is generally higher than non-vegetarians. Post-menopausal status was defined as women who were into menopause at least one year from their last menstruation at the beginning of the study. Subjects were asked to continue their habitual diet and to maintain their pre-study physical activities and body weights. Study protocol and informed consent were approved by the Human Subjects Protection Committee at San Francisco State University.
Administration of psyllium fiber
Subjects were asked to consume psyllium fiber enriched cookies for 6 weeks. We incorporated 15 g of psyllium fiber into cookies (≈5 g/cookie). Subjects consumed 3 cookies/d (one in each meal). Psyllium fiber enriched cookies were made in the departmental food laboratory, according to the formulation given in Table 1. Previous studies documented that 4–6 week feeding period was adequate to observe changes in serum lipids with psyllium fiber intake [17, 18]. The reason that psyllium fiber was incorporated into cookies rather than administering as supplements because fiber has shown to produce maximum cholesterol-lowering effect when it is taken mixed with foods . Numerous products (muffins and powdered drink mixes) incorporating psyllium were tested for palatability and cookies yielded the most appetizing product. Cookies were individually supplied to subject's home in a fresh or frozen form on a weekly basis. The rationale for the amount of fiber used (15 g/d) was based on the recommendation of 25–35 g/d of fiber intake for adults . Since Americans consume about 15 g/d of fiber , by adding 15 g/d subjects would meet the recommendations for fiber. During the study, subjects were asked to refrain from consuming fiber supplements. If a subject was a social drinker that subject was asked to maintain her usual alcohol intake level.
Serum lipid analysis
Twelve-hour overnight fasting blood samples were obtained from venipuncture in the arm at the beginning and at the end of the 6-week period by a certified phlebotomist. Blood samples were centrifuged and serum was separated before lipid analysis. Serum samples were analyzed for total cholesterol, LDL-cholesterol, HDL-cholesterol, triglycerides, apolipoprotein A-1, and apolipoprotein B using an automatic analyzer (Quest Diagnostic Laboratories, Dublin, CA).
Data were presented as mean ± standard deviation. Paired t-test was used to determine the significant difference between the baseline and the post-fiber treatment values in pre- and post-menopausal women (Microsoft Excel for Windows, 1999). Statistical significance was set at P < 0.05.
Of 25 subjects, 19 (8 pre- and 11 post-menopausal) women completed the study. Reasons for discontinuation of participation included gastrointestinal discomfort, increased frequency of bowel movements, softer stools, and personal travel and time conflicts. Subjects' characteristics were summarized in Table 2. As expected, the post-menopausal women were significantly older compared to pre-menopausal women (p < 0.05). Body mass index (BMI) at baseline between pre- and post-menopausal women was not significantly different. BMI for all subjects were within the desirable low health risk range. There were no significant differences in body weight between baseline to post-study measurements in both pre- and post-menopausal women (data are not shown).
The baseline and post-study serum lipid concentrations for pre- and post-menopausal women are presented in Table 3. Mean serum total cholesterol concentration with psyllium fiber intake was significantly lower compared to baseline values in post-menopausal women (218.7 mg/dL vs. 230.8 mg/dL; p < 0.05) but not in pre-menopausal women (240.5 mg/dL vs. 243.6 mg/dL). The decrease in post-menopausal women with respect to total serum cholesterol was ≈5.2%.
Mean HDL cholesterol was significantly lower in post-menopausal women with fiber intake (65 mg/dL to 58.4 mg/dL) (p < 0.05). In contrast, no change was observed in HDL-cholesterol concentrations in pre-menopausal women with psyllium. Changes in LDL-cholesterol in pre- and post-menopausal women from baseline to post-study were not significant although there was a slight decline in values with psyllium fiber intake. The decline was ≈4.6% in post-menopausal women and was ≈3.2% in pre-menopausal women.
There was a ≈7.6% and ≈18.6% increase in serum triglycerides in pre- and post-menopausal women, respectively, with psyllium fiber supplementation. However, these changes were not statistically significant. Similarly, there were no significant changes in response to psyllium fiber intake in serum apolipoprotein A-1 and apolipoprotein B concentrations in both pre- and post-menopausal women.
We investigated the effect of psyllium fiber intake at 15 g/d dosage level for 6 weeks on serum lipids in free-living, pre- and post-menopausal, hypercholesterolemic women. Psyllium fiber significantly lowered serum total cholesterol in post-menopausal women. Decreased total serum cholesterol was primarily due to changes in the HDL-cholesterol. Although LDL-cholesterol was lower (≈4.6%) with fiber intake, the decrease was not statistically significant when compared to baseline values in post-menopausal women. Concentrations of serum triglycerides, apolipoprotein A-1, and apolipoprotein B were not affected by psyllium fiber intake in post-menopausal women. Also, psyllium fiber intake had no effect on serum total cholesterol, LDL-cholesterol, HDL-cholesterol, triglycerides, apolipoprotein A-1, and apolipoprotein B in pre-menopausal, hypercholesterolemic women.
Previously, we documented cholesterol-lowering effect of psyllium in normocholesterolemic humans . Other studies have shown that consumption of psyllium fiber reduced serum total cholesterol and LDL-cholesterol in hypercholesterolemic subjects [12, 13]. Several other investigators reported a 3–17% reduction of serum total cholesterol and a 4–20% reduction of LDL-cholesterol with psyllium fiber [12, 19, 21, 22]. In a meta-analysis, Anderson et al. (2000b) reported that consumption of 10.2 g psyllium/d lowered total cholesterol by 4%. Whereas, in our study, we achieved a ≈5.2% decrease in total serum cholesterol concentrations at a dosage level of 15 g psyllium/d. Most of the psyllium fiber used in previous studies was either in the form of ready-to-eat cereal or as fiber supplement. In our study, we administered psyllium fiber in cookies. Thus, the method of administration of psyllium might account for some differences in cholesterol-lowering property of psyllium fiber in various studies.
Davidson et al.  reported that consumption of psyllium fiber lowered total cholesterol without modifying HDL-cholesterol concentrations. In contrast, our study did not reveal that psyllium fiber reduced serum total cholesterol without lowering HDL-cholesterol. The discrepancy in results between this study and other studies may likely be due to differences in characteristics of subjects. Also, our subjects consumed self-selected diets during the study. Thus, the diet intake might have some influence on the study outcomes.
In this study, we found the hypocholesterolemic effect of psyllium fiber in post-menopausal women but not in pre-menopausal women indicating that pre- and post-menopausal women responded differently to psyllium fiber. Lack of significant hypocholesterolemic effect of psyllium fiber in pre-menopausal women suggests that the elevated cholesterol concentrations in those subjects might be due to genetic causes and thus are unresponsive to dietary modification. On the other hand, elevated cholesterol in post-menopausal women is often due to deranged lipid metabolism (non-genetic causes) arising from loss of estrogen function and thus is responsive to psyllium fiber intervention. In post-menopausal women with established CVD, higher intake of fiber has been associated with less progression of coronary atherosclerosis . Also, psyllium fiber significantly reduced post-prandial concentrations of serum triglycerides, glucose, and insulin in persons with diabetes  suggesting a role for psyllium fiber in the management of diabetes.
Previous studies on the effect of psyllium fiber on triglycerides yielded equivocal results [22, 25, 26]. Psyllium fiber intake significantly reduced plasma triglycerides in men, but in post-menopausal women, it resulted in a significant increase with no change in pre-menopausal women . In our study, we found no significant effect of psyllium fiber intake on serum triglycerides in both pre- and post-menopausal women, although there was a trend towards increased serum triglycerides.
The lack of effect of psyllium fiber intake on serum lipids in pre-menopausal women might be due insufficient number of subjects recruited to observe statistically significant differences. Large sample size might reveal significant total cholesterol and/or LDL-cholesterol reduction. Therefore, the results of this study must be used with caution. Although, we have instructed the subjects not to alter their dietary habits during the study, it is possible that our results may have confounded by the fiber from unintended food sources. Because psyllium fiber was incorporated into cookies, consumption of psyllium enriched cookies lead to an additional intake of ≈30 g/d fat. This may have blunted the hypocholesterolemic effect of psyllium in both pre- and post-menopausal women. Additionally, all subjects in the post-menopausal group were Asians. It is not known how this has affected the cholesterol-lowering effect of psyllium.
In conclusion, post- and pre-menopausal women differed with regard to lipid responses to psyllium fiber. Serum total cholesterol was responsive to psyllium fiber in post-menopausal women. Post-menopausal women would likely benefit from addition of psyllium fiber to their diets in reducing the risk of heart diseases because every 1% reduction in serum total cholesterol concentration results in 2% reduction in risk for heart diseases . Differences in cholesterol-lowering response to psyllium intake may be related to the differences in hormonal status between pre- and post-menopausal women [15, 26]. Additionally, consumption of psyllium fiber provides a low-cost adjunct to the National Cholesterol Education Program diets for hypercholesterolemia.
National Cholesterol Education Program, Adult Treatment Panel III: Third report of the Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults. [http://www.nhlbi.nih.gov/guidelines/cholesterol/atp3upd04.htm]
Schaefer EJ: Lipoproteins, nutrition, and heart disease. Am J Clin Nutr. 2002, 75: 191-212.
Matthews KA, Meilahn E, Kuller LH, Kelesey SF, Caggiula AW, Wing RR: Menopause and risk factors for coronary heart disease. N Engl J Med. 1989, 321: 641-646.
McNamara JR, Campos H, Ordovas : Effect of gender, age, and lipid status on low density lipoprotein sub fraction distribution. Results from the Framingham offspring study. Arteriosclerosis. 1987, 7: 483-490.
Seed M: Postmenopausal hormone replacement therapy, coronary heart disease and plasma lipoproteins. Drugs. 1994, 47: 25-34. 10.2165/00003495-199400472-00006.
Walsh A, Ito Y, Breslow JL: Apolipoprotein A-a gene expression in transgenic mice. Biotechnol. 1991, 16: 227-235.
Nelson HD, Humphrey LL, Nygren P, Teutsch SM, Allan JD: Postmenopausal hormone replacement therapy. JAMA. 2002, 288: 872-881. 10.1001/jama.288.7.872.
Connor WE, Connor SL: Importance of diet in the treatment of familial hypercholesterolemia. Am J Cardiol. 1993, 72: 42D-53D. 10.1016/0002-9149(93)90010-A.
Goldman L, Garber AM, Grover SA: 27th Bethesda Conference: matching the intensity of risk factor management with the hazard for coronary heart disease events. Task force 6. Cost effectiveness of assessment and management of risk factors. J Am Coll Cardiol. 1996, 27: 1020-1030. 10.1016/0735-1097(96)87734-5.
Davidson MR, Maki KC, Kong IC, Dugan LD, Tprro SA, Hall HA, Drennan KB, Anderson SM, Fulgoni VL, Saldanha LG, Olson BH: Long-term effects of consuming foods containing psyllium seed husk on serum lipids in subjects with hypercholesterolemia. Am J Clin Nutr. 1998, 67: 367-376.
Ganji V, Betts N: Fat, cholesterol, fiber and sodium intakes of US population: evaluation of diets reported in 1987–88 Nationwide Food Consumption Survey. Eur J Clin Nutr. 1995, 49: 915-920.
Brown L, Rpsmer B, Willett W, Sacks FM: Cholesterol-lowering effects of dietary fiber: a meta-analysis. Am J Clin Nutr. 1999, 69: 30-42.
Anderson JW, Allgood LD, Lawrence A, Altringer LA, Ierdack GR, Hengehold DA: Cholesterol-lowering effects of psyllium intake adjunctive to diet therapy in men and women with hypercholesterolemia: meta-analysis of 8 controlled trials. Am J Clin Nutr. 2000, 71: 472-479.
Moreyra AE, Wilson AC, Koraym A: Effect of combining psyllium fiber with simvastatin in lowering cholesterol. Arch Intern Med. 2005, 165: 1161-1166. 10.1001/archinte.165.10.1161.
Vega-Lopez S, Conde-Knape K, Vidal-Quintanar RL, Shachter NS, Fernandez ML: Sex and hormonal status influence the effects of psyllium on lipoprotein remodeling and composition. Metabolism. 2002, 51: 500-507. 10.1053/meta.2002.31342.
Jenkins DA, Wolever TMS, Rao AV, Hegele RA, Mitchell SJ, Ransom TP, Boctor DL, Spadafora PJ, Jenkins AL, Mehling C: Effect on blood lipids of very high intakes of fiber in diets low in saturated fat and cholesterol. N Eng J Med. 1993, 329: 21-26. 10.1056/NEJM199307013290104.
Anderson JW, Riddell-Mason S, Gustafson NJ, Smith SF, Mackery M: Cholesterol-lowering effects of psyllium-enriched cereal as an adjunct to a prudent diet in the treatment of mild to moderate hypercholesterolemia. Am J Clin Nutr. 1992, 56: 93-98.
Ganji V, Kies CV: Psyllium husk fiber supplementation to the diets rich in soybean or coconut oil: hypocholesterolemic effect in healthy humans. lnt J Food Sci Nutr. 1996, 47: 103-110. 10.3109/09637489609012571.
Wolever TM, Jenkins DJ, Mueller S, Boctor DL, Ransom TP, Patten R, Chao ES, McMillam K, Fulgoni VL: Method of administration influences the serum cholesterol-lowering effect of psyllium. Am J Clin Nutr. 1994, 59: 1055-1059.
Lee DL, Nieman DC: Nutritional Assessment. 1996, 47-53. 2
Olson BH, Anderson SM, Becker MP, Anderson JW, Hunninghake DB, Jenkins DJ, LaRosa JC, Rippe JM, Roberts DC, Stoy DB, Summerball CD, Truswell AS, Wolever TM, Morris DH, Fulgoni VL: Psyllium-enriched cereals lower blood total cholesterol and LDL cholesterol, but not HDL cholesterol, in hypercholesterolemic adults: results of a meta-analysis. J Nutr. 1997, 127: 1973-1980.
Anderson JW, Davidson MH, Blonde L, Brown WV, Howard WJ, Ginsberg H, Allgood LD, Weingand KW: Long-term cholesterol-lowering effects of psyllium as an adjunct to diet therapy in the treatment of hypercholesterolemia. Am J Clin Nutr. 2000, 71: 1433-1488.
Erkkila AT, Herrington DM, Mozaffarian D, Lichtenstein AH: Cereal fiber and whole grain intake are associated with reduced progression of coronary-artery atherosclerosis in postmenopausal women with coronary artery disease. Am Heart J. 2005, 150: 94-101. 10.1016/j.ahj.2004.08.013.
Rodriguez-Moran M, Guerrero-Romero F, Lazcano-Burciago G: Lipid- and glucose-lowering efficacy of Plantago Psyllium in type II diabetes. J Diabetes Complications. 1998, 12: 273-278. 10.1016/S1056-8727(98)00003-8.
Bell LP, Hectorne K, Reynolds H, Balm TK, Hunninghake DB: Cholesterol-lowering effects of psyllium hydrophilic mucilloid. Adjunct therapy to a prudent diet for patients with mild to moderate hypercholesterolemia. JAMA. 1989, 261: 3419-3423. 10.1001/jama.261.23.3419.
Vega-Lopez S, Vidal-Quintanar RL, Fernandez ML: Sex and hormonal status influence plasma lipid responses to psyllium. Am J Clin Nutr. 2001, 74: 435-441.
Roussouw JE, Rifkind BM: Does lowering serum cholesterol lower coronary heartdisease risk?. Endocrinol Metab Clin N Am. 1990, 19: 279-297.
The study was supported by the Zellemer Grant program from the California Dietetic Association.
The authors declare that they have no competing interests.
VG and JK directed the research. VG was responsible for getting the support for the project. Both authors are responsible for collecting the data, analysis of data, and drafting the manuscript. All authors read and approved the manuscript before submission.
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Ganji, V., Kuo, J. Serum lipid responses to psyllium fiber: differences between pre- and post-menopausal, hypercholesterolemic women. Nutr J 7, 22 (2008). https://doi.org/10.1186/1475-2891-7-22
- Serum Lipid
- Serum Total Cholesterol
- Fiber Intake
- Hypocholesterolemic Effect