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Effects of high-protein vs. high- fat snacks on appetite control, satiety, and eating initiation in healthy women
© Ortinau et al.; licensee BioMed Central Ltd. 2014
Received: 1 August 2014
Accepted: 23 September 2014
Published: 29 September 2014
The purpose of this study was to determine whether a high-protein afternoon yogurt snack improves appetite control, satiety, and reduces subsequent food intake compared to other commonly-consumed, energy dense, high-fat snacks.
Twenty, healthy women (age: 27 ± 2 y; BMI: 23.4 ± 0.7 kg/m2) completed the randomized crossover design study which included 3, 8-h testing days comparing the following 160 kcal afternoon snacks: high-protein yogurt (14 g protein/25 g CHO/0 g fat); high-fat crackers (0 g protein/19 g CHO/9 g fat); and high-fat chocolate (2 g protein/19 g CHO/9 g fat). Participants were acclimated to each snack for 3 consecutive days. On day 4, the participants consumed a standardized breakfast and lunch; the respective snack was consumed 3-h post-lunch. Perceived hunger and fullness were assessed throughout the afternoon until dinner was voluntarily requested. An ad libitum dinner was then provided. The consumption of the yogurt snack led to greater reductions in afternoon hunger vs. chocolate (p < 0.01). No differences in afternoon fullness were detected. The yogurt snack also delayed eating initiation by approximately 30 min compared to the chocolate snack (p < 0.01) and approximately 20 min vs. crackers (p = 0.07). The yogurt snack led to approximately 100 fewer kcals consumed at dinner vs. the crackers (p = 0.08) and chocolate (p < 0.05). No other differences were detected.
These data suggest that, when compared to high-fat snacks, eating less energy dense, high-protein snacks like yogurt improves appetite control, satiety, and reduces subsequent food intake in healthy women.
Over the past 30 years, there has been a significant increase in the number of snacking occasions in the US, which has occurred concomitantly with the rise in obesity [1, 2]. The relationship between increased snacking and obesity may well be attributed to the types of foods typically consumed in these smaller ‘in-between meal’ eating occasions. In the US population, nearly one third of daily intake is comprised of snack foods which tend to be nutrient-poor, yet energy dense foods (i.e., desserts, salty/high fat snacks, and candy) that are high in saturated fat and/or simple sugars and may lead to energy surplus/over-eating [1, 3]. However, limited data exist regarding whether the replacement of energy dense, high-fat snacks with ‘healthier’ alternatives has a beneficial effect on food intake regulation.
Two well-established dietary factors that consistently improve appetite control, satiety, and/or reduce daily food intake include the consumption of low energy dense foods  and increased dietary protein . Recent data from our lab demonstrated that the consumption of a less energy dense, higher protein yogurt snack led to reduced post-snack hunger, increased post-snack fullness, and delayed eating initiation compared to yogurts that were lower in protein content and higher in energy density . Thus, we sought to extend the previous findings to examine whether the consumption of a less energy dense, high-protein yogurt snack leads to greater appetite control, satiety, and reductions in subsequent food intake compared to other commonly consumed snacks that are energy-dense and high in fat.
Twenty pre-menopausal women were recruited through flyers posted on the University of Missouri campus or through the University’s email listserv. Participants were healthy, non-smoking women (age: 27 ± 2 y; BMI: 23.4 ± 0.7 kg/m2) who had no food allergies, eating disorders, diabetes, recent rapid weight loss/gain, not on medication that would alter appetite, and followed a typical eating pattern including 3 meals/day and an afternoon snack. All participants were informed of the study objectives, procedures, and potential risks. Written consent was obtained from all participants. The study procedures were approved by the University of Missouri’s Human Subjects Institutional Review Board.
6 oz. Cup
Energy Content (kcal (kJ))
Energy Density (kcal/g)
Total Protein (g)
Total Carbohydrates (g)
Total Fat (g)
Snack Palatability (mm)
70 ± 10
80 ± 5
80 ± 10
Data and statistical analyses
Summary statistics (sample means, net incremental area under the curve (AUC), and/or SEM) were computed for all data. A repeated measures ANOVA was applied to compare the main effects of snacking on perceived sensations, time to dinner request, and dinner energy content. When main effects were detected, post hoc analyses were performed using Least Significant Difference procedures to identify differences between treatments. All analyses were conducted using the Statistical Package for the Social Sciences (SPSS; version 21; Chicago, IL).
The consumption of a less energy dense, high-protein yogurt snack led to greater reductions in afternoon hunger, delayed the onset of eating, and reduced food intake at the dinner meal compared to energy dense, high-fat snacks including crackers and/or chocolate. These data suggest that eating less energy dense, high-protein foods like yogurt improves appetite control, satiety, and reduces short-term food intake in women.
A macronutrient hierarchy exists in which the satiety effects of foods can be attributed, in part, to their nutritional composition with the consumption of dietary fat having the lowest satiety effect and protein displaying the greatest effect [9–11]. Another closely-linked dietary factor that has strong satiety properties includes the energy density of the foods . Studies by Rolls et al. [4, 12, 13] consistently illustrate increased satiety and reduced food intake when consuming less energy dense foods compared to more energy dense foods. Since high-protein foods are typically less energy dense than high-fat foods, it is difficult to tease out the independent effects of macronutrient content and energy density. However, we have previously shown that, when matched for energy density, the consumption of higher protein meals lead to improved appetite control and satiety compared to normal protein versions .
Several previous snack studies have also examined the combined effects of reduced energy density and increased dietary protein [6, 15, 16]. Specifically, Marmonier et al.  included normal weight men and provided 240 kcal snacks which varied in macronutrient content and energy density. Although afternoon hunger and fullness were not different between snacks, the consumption of the low energy dense, high-protein snack delayed eating by 35 min compared to the high energy dense, high-fat snack (p < 0.05) and 25 min compared to a moderate energy dense, high-carbohydrate snack (p < 0.05). However, no difference in dinner intake was observed. In a study by Chapelot et al.  that included normal weight men, the consumption of a 285 kcal less energy dense, high-protein liquid yogurt snack led to a greater increase in post-snack fullness and greater decrease in post-snack hunger compared to a energy dense high-fat chocolate snack. However, eating initiation and dinner intake were not different. Lastly, in our previous study in normal weight women, we examined the effects of 160 kcal afternoon yogurt snacks, varying in energy density and macronutrient content. The less energy dense, high-protein yogurt led to reduced hunger, increased fullness, and delayed subsequent eating compared to the energy dense, high-carbohydrate version . The inconsistent findings of eating initiation and diner intake between the previous studies and the current study may be attributed to the differences in snack type, macronutrient content, energy content, and energy density. However, it is important to note that, despite these differences, none of the studies showed a negative effect of consuming a less energy dense, higher protein snack in the afternoon. Further research is necessary to comprehensively identify the effects of less energy dense, protein snacks on energy intake regulation.
We sought to compare the satiety effects following the consumption of commercially-available, commonly consumed afternoon snacks. In using this approach, we were unable to tightly control macronutrient quantity and quality. Thus, although both high-fat snacks were similar in carbohydrate content (19 g/snack) and total fat content (9g/snack), they varied in carbohydrate and fatty acid composition. Specifically, the chocolate snack contained mostly simple carbohydrates (18 g), whereas the crackers included only 2 g of simple carbohydrates. In addition, the chocolate snack was high in saturated fat (5.5 g) and low in polyunsaturated fat (0.4 g), whereas the crackers contained only 1.5 g of saturated fat but 5.0 g of polyunsaturated fat. Some, but not all studies, have demonstrated greater reductions in hunger and/or greater increases in satiety following the consumption of complex vs. simple carbohydrates  and after the consumption of saturated vs. polyunsaturated fatty acids . In the current study, no differences in hunger, satiety, or subsequent food intake were detected between the crackers and chocolate, suggesting that the higher saturated fatty acid content of the chocolate may have negated the negative effects of the simply carbohydrates. Collectively, these examples illustrate the need to control, not only the macronutrient content, but the type/quality as well. Other limitations include the limited assessment of only including perceived sensations of hunger and satiety in normal weight, adult women. Thus, further research incorporating both the appetitive and hormonal signals involved with energy intake regulation in overweight and/or obese individuals is warranted. Lastly, this was an acute trial over the course of a single day. Longer-term randomized controlled trials are also critical in establishing whether the daily consumption of a less energy dense, high-protein snack improves body weight management.
The less energy dense, high-protein yogurt snack induced satiety and reduced subsequent food intake compared to other commonly consumed snacks, specifically energy dense, high-fat crackers and chocolate. These findings suggest that a less energy dense, high-protein afternoon snack could be an effective dietary strategy to improve appetite control and energy intake regulation in healthy women.
General Mills Bell Institute of Health and Nutrition supplied the funds to complete the study but was not involved in the design, implementation, analysis, or interpretation of data.
- Piernas C, Popkin BM: Snacking increased among U.S. adults between 1977 and 2006. J Nutr. 2010, 140: 325-332. 10.3945/jn.109.112763.View ArticlePubMedPubMed CentralGoogle Scholar
- Piernas C, Popkin BM: Increased portion sizes from energy-dense foods affect total energy intake at eating occasions in US children and adolescents: patterns and trends by age group and sociodemographic characteristics, 1977–2006. Am J Clin Nutr. 2011, 94: 1324-1332. 10.3945/ajcn.110.008466.View ArticlePubMedPubMed CentralGoogle Scholar
- Duffey KJ, Popkin BM: Energy density, portion size, and eating occasions: contributions to increased energy intake in the United States, 1977–2006. PLoS Med. 2011, 8: e1001050-10.1371/journal.pmed.1001050.View ArticlePubMedPubMed CentralGoogle Scholar
- Rolls BJ: The relationship between dietary energy density and energy intake. Physiol Behav. 2009, 97: 609-615. 10.1016/j.physbeh.2009.03.011.View ArticlePubMedPubMed CentralGoogle Scholar
- Leidy HJ: Increased dietary protein as a dietary strategy to prevent and/or treat obesity. Mo Med. 2014, 111: 54-58.PubMedGoogle Scholar
- Douglas SM, Ortinau LC, Hoertel HA, Leidy HJ: Low, moderate, or high protein yogurt snacks on appetite control and subsequent eating in healthy women. Appetite. 2013, 60: 117-122.View ArticlePubMedGoogle Scholar
- Flint A, Raben A, Blundell JE, Astrup A: Reproducibility, power and validity of visual analogue scales in assessment of appetite sensations in single test meal studies. Int J Obes Relat Metab Disord. 2000, 24: 38-48. 10.1038/sj.ijo.0801083.View ArticlePubMedGoogle Scholar
- Ortinau LC, Culp JM, Hoertel HA, Douglas SM, Leidy HJ: The effects of increased dietary protein yogurt snack in the afternoon on appetite control and eating initiation in healthy women. Nutr J. 2013, 12: 71-10.1186/1475-2891-12-71.View ArticlePubMedPubMed CentralGoogle Scholar
- Holt SH, Miller JC, Petocz P, Farmakalidis E: A satiety index of common foods. Eur J Clin Nutr. 1995, 49: 675-690.PubMedGoogle Scholar
- Stubbs RJ, van Wyk MC, Johnstone AM, Harbron CG: Breakfasts high in protein, fat or carbohydrate: effect on within-day appetite and energy balance. Eur J Clin Nutr. 1996, 50: 409-417.PubMedGoogle Scholar
- Westerterp-Plantenga MS, Rolland V, Wilson SA, Westerterp KR: Satiety related to 24 h diet-induced thermogenesis during high protein/carbohydrate vs high fat diets measured in a respiration chamber. Eur J Clin Nutr. 1999, 53: 495-502. 10.1038/sj.ejcn.1600782.View ArticlePubMedGoogle Scholar
- Blatt AD, Williams RA, Roe LS, Rolls BJ: Effects of energy content and energy density of pre-portioned entrees on energy intake. Obesity (Silver Spring). 2012, 20: 2010-2018. 10.1038/oby.2011.391.View ArticleGoogle Scholar
- Williams RA, Roe LS, Rolls BJ: Assessment of satiety depends on the energy density and portion size of the test meal. Obesity (Silver Spring). 2014, 22: 318-324. 10.1002/oby.20589.View ArticleGoogle Scholar
- Leidy HJ, Ortinau LC, Douglas SM, Hoertel HA: Beneficial effects of a higher-protein breakfast on the appetitive, hormonal, and neural signals controlling energy intake regulation in overweight/obese, “breakfast-skipping,” late-adolescent girls. Am J Clin Nutr. 2013, 97: 677-688. 10.3945/ajcn.112.053116.View ArticlePubMedPubMed CentralGoogle Scholar
- Marmonier C, Chapelot D, Louis-Sylvestre J: Effects of macronutrient content and energy density of snacks consumed in a satiety state on the onset of the next meal. Appetite. 2000, 34: 161-168. 10.1006/appe.1999.0302.View ArticlePubMedGoogle Scholar
- Chapelot D, Payen F: Comparison of the effects of a liquid yogurt and chocolate bars on satiety: a multidimensional approach. Br J Nutr. 2010, 103: 760-767. 10.1017/S000711450999225X.View ArticlePubMedGoogle Scholar
- Aller EE, Abete I, Astrup A, Martinez JA, van Baak MA: Starches, sugars and obesity. Nutrients. 2011, 3: 341-369. 10.3390/nu3030341.View ArticlePubMedPubMed CentralGoogle Scholar
- Kozimor A, Chang H, Cooper JA: Effects of dietary fatty acid composition from a high fat meal on satiety. Appetite. 2013, 69: 39-45.View ArticlePubMedGoogle Scholar
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