- Open Access
- Open Peer Review
This article has Open Peer Review reports available.
Associations between body composition and nutritional assessments and biochemical markers in patients with chronic radiation enteritis: a case–control study
© Cai et al. 2016
Received: 23 March 2016
Accepted: 24 May 2016
Published: 28 May 2016
Chronic radiation enteritis (CRE) is defined as loss of absorptive capacity after irradiation due to chronic inflammation and damage of intestinal mucosa, which may lead to varying degrees of malnutrition. The aim of this study was to evaluate the potential correlation between the nutritional status and systemic inflammation in patients with CRE.
Medical records of 92 patients with CRE and 184 age- and sex-matched controls in a single center from January 2010 to October 2015 were retrospectively reviewed. All enrolled subjects underwent nutritional status analysis, including three different nutritional indices: Nutritional Risk Screening-2002 (NRS-2002), Patient-generated Subjective Global Assessment (PG-SGA) and Controlling Nutritional Status (CONUT), bioelectrical impedance spectroscopy (BIS), and biochemical markers, within 24 h of admission.
The results showed that NRS-2002, PG-SGA and CONUT were all positively correlated with neutrophil/lymphocyte ratio (NLR) (r = 0.304, 0.384 and 0.425, all p < 0.001) and C-reactive protein (CRP) (r = 0.357, 0.479 and 0.230, all p < 0.001), while negatively correlated with albumin (r = −0.612, −0.727 and −0.792, all p < 0.001) and total cholesterol (TC) (r = −0.485, −0.545 and −0.473, all p < 0.001) in patients with CRE, respectively. Body cell mass (BCM) has been deemed a key body composition parameter. It was positively correlated with albumin (r = 0.489, p < 0.001) and TC (r = 0.237, p < 0.001), while negatively correlated with NLR (r = −0.140, p = 0.02) and CRP (r = −0.215, p < 0.001). A multivariate linear regression analysis showed that values of intracellular water (β coefficient = 0.760, p < 0.001), extracellular water (β coefficient = 0.006, p = 0.011), protein (β coefficient = 0.235, p < 0.001) and CRP (β coefficient = 0.001, p = 0.009) were independent determinants of BCM.
This study revealed that BIS combined with nutritional assessments and biochemical markers were appropriate methods to assess the nutritional and inflammatory status in patients with CRE. Furthermore, the nutritional status was verified to be significantly correlated with systemic inflammation.
Radiation enteritis (RE) is defined as inflammation and damage of intestinal mucosa by short exposure to radiation at the abdomen and pelvis level, which can be subdivided into acute (ARE) and chronic radiation enteritis (CRE). ARE mostly occurs days or weeks after radiation therapy and CRE may emerge after several years or even decades [1, 2]. CRE results from obliterative endarteritis which progresses to intestinal wall thickening, ulceration and fibrosis, leading to intestinal stricture, fistula and even perforation. Because of insufficient intestinal mucosa for nutrition absorption, most CRE patients suffer from mild, moderate or severe malnutrition . The volume of small bowel affected and total radiation dose are the most important factors of the risks of acute and late toxicity. Acute inflammation may resolve and develop into a more chronic state with arteriolar endarteritis and fibrosis. This progressive vasculitis causes intestinal ischemia that in turn leads to mucosal friability and neovascularization as well as an exaggerated submucosal fibrosis.
According to the consensus updated, nutrition diagnoses could be categorized into three species: 1. Starvation-related malnutrition, which is chronic malnutrition without inflammation; 2. Chronic disease-related malnutrition, where inflammation is chronic and of mild to moderate degree; 3. Acute disease and injury-related malnutrition, where inflammation is acute and severe [4, 5]. Irrespective of disease severity, inflammation always plays a vital role in malnutrition [6, 7]. Based on the aforementioned nutritional definitions, the nutritional status of CRE is presumed to be classified as chronic disease-related malnutrition, where inflammation is chronic and of mild to moderate degree.
Evaluation of the correlation between the nutritional status and systemic inflammation was well conducted in chronic undernourished patients . Nevertheless its effect has not yet been validated in patients with CRE. The objective of this study was to evaluate the potential correlation between the nutritional status and systemic inflammation in patients with CRE.
Protocol and participants
Clinical records of patients admitted to our center with CRE as a definite diagnosis from January 2010 to October 2015 were retrospectively reviewed. The diagnoses depended on the combination of clinical symptoms, medical histories and the exclusion of other potential diagnoses. Clinical manifestations included weight loss, abdominal pain, rectal bleeding, diarrhea, malabsorption, stricture, intestinal obstruction and even atraumatic perforation [1, 2, 9]. Gastrointestinal endoscopy was most useful in excluding other causes such as infection and recurrent neoplasia . Moreover, imaging scans have been used to support a suspected diagnosis. Magnetic resonance enterography has shown focal abnormalities generally in the distal ileum of CRE patients .
Most patients presented with symptoms of partial intestinal obstruction, bleeding or fistula at least 6 months after radiotherapy. Given the poor nutritional status, preoperative nutrition support by parenteral nutrition or enteral nutrition was provided. All patients underwent body composition analysis assessed by bioelectrical impedance spectroscopy (BIS) before surgery. Patients were matched 1:2 by age and sex with healthy people who had the medical examination in our center. Patients with ARE, tumor recurrence and concomitant chronic diseases such as diabetes, chronic renal failure and liver cirrhosis were excluded. Furthermore, patients with incomplete information due to physical, cognitive or mental problems were also excluded. Data regarding nutritional assessments, BIS and biochemical markers were extracted for stepwise analysis. The baseline demographic characteristics of subjects enrolled were also reviewed.
The study was conducted in accordance with the Declaration of Helsinki and approved by the Institutional Ethical Committee of Jinling hospital. All patients gave their informed consent prior to entering the study.
Nutritional Risk Screening-2002 (NRS-2002) Questionnaire was scored in each of three components, including nutritional status, severity of disease and age . Patient-Generated Subjective Global Assessment (PG-SGA) comprised of two sections that were completed by patients and clinicians, respectively. Patients were required to fulfill the form included weight loss, intake, symptoms and functional capacity initially. Afterwards, clinicians assessed the disease status and its relation with metabolic demands, nutritional requirements and physical examinations. Patients were classified based on a total score of two sections summed and a higher score reflected a higher risk of malnutrition . The Controlling Nutritional Status (CONUT) score was based on the method developed by Ulibarri et al. . The score was calculated by three parameters, including serum albumin, total cholesterol level and total lymphocyte count. All these nutritional assessments were implemented within the first 24 h of hospital admission and the results were analyzed retrospectively.
Anthropometric measurements and body composition analysis
Body composition was measured by BIS, a selective approach of bioelectrical impedance analysis (BIA). When weight and height are measured, patients were required to run the test after meal for at least 2 h with barefoot and underwear. Body mass index (BMI) was calculated as on weight in kilogram divided by height in meters squared (kg/m2). Then the patient lay down fully relaxed with limbs abducted from the body. After removing electrodes with alcohol cotton, attach them at each hand wrist and foot ankle to make sure the electrode contact area is beyond 5 cm2. The instrument used was multi-frequency electrical impedance analyzer (Inbody S20, Korea) with frequency ranging from 1 kHz to 1 MHz and 800A constant electrical flow through the body to measure the impedance value. The whole process took only less than 2 min. All data were stored within the instrument and analyzed by computer software automatically. Body composition parameters including fat-free mass (FFM), fat mass (FM), body cell mass (BCM), total body water (TBW), extracellular water (ECW), intracellular water (ICW), protein and mineral were collected.
Biochemical markers analysis
Blood samples (5 mL) were collected from a peripheral vain with a single puncture early in the morning. Biochemical markers included albumin, neutrophil count, lymphocyte count, C-reactive protein (CRP) and total cholesterol (TC) were measured using an automated biochemical analyzer (Olympus AU400 Chemistry Analyzer, Tokyo, Japan). Neutrophil/lymphocyte ratio (NLR) was calculated from these parameters.
Statistical analysis was performed using SPSS version 20.0 (SPSS, Inc, an IBM Company, Chicago, IL). The results of analysis were expressed as mean ± SD. Data regarding patients with CRE and matched controls were compared with student’s t test. The Pearson’s product–moment correlation coefficient was used to assess correlation of two variables. A multivariate linear regression analysis with stepwise selection of covariates was used to explore the correlation between BCM and other variables. Only factors that were statistical different could be selected into the stepwise multivariate regression analysis. All candidate variables were checked to see if their significance has been reduced below the specific level. If a non-significant variable was found, it is removed from the analysis. Both p < 0.01 and p < 0.05 was recognized as statistically significant. The compound bar chart was used to describe body composition parameters of patients compared with controls. The scatter plot was used to describe correlations between BCM and biochemical markers in patients with CRE.
Demographic characteristics, nutritional assessments and biochemical markers
Demographic characteristics, nutritional assessments and biochemical indices of patients with CRE compared with controls
CRE (n = 92)
Control (n = 184)
53 ± 11.54
52.98 ± 11.51
65 females/27 males
130 females/54 males
161.96 ± 8.06
161.21 ± 7.28
45.68 ± 8.68
63.79 ± 11.55
17.39 ± 2.67
24.44 ± 3.42
2.55 ± 0.97
0.26 ± 0.69
14.18 ± 3.25
2.01 ± 0.82
2.05 ± 1.91
36.61 ± 5.56
46.40 ± 2.58
3.36 ± 2.28
1.84 ± 0.78
11.32 ± 9.98
2.77 ± 3.80
3.70 ± 0.99
5.25 ± 1.08
Body composition analysis
Body composition parameters of patients with CRE compared with controls
CRE (n = 92)
Control (n = 184)
16.66 ± 3.34
22.44 ± 4.28
11.51 ± 2.25
11.06 ± 2.05
28.13 ± 5.41
33.51 ± 6.26
38.18 ± 7.38
45.50 ± 8.43
7.45 ± 4.77
18.30 ± 5.64
23.86 ± 4.77
31.42 ± 5.99
7.16 ± 1.48
8.98 ± 1.71
2.88 ± 0.64
3.01 ± 0.45
Correlations between nutritional assessments and biochemical markers in patients with CRE
Correlations between nutritional assessments and biochemical markers in patients with CRE
Correlations between body composition parameters and biochemical markers in patients with CRE
Correlations between body composition parameters and biochemical markers in patients with CRE
Correlations between BCM and multiple variables
A multivariate linear regression analysis of BCM
The present study revealed that values of body composition parameters and all nutritional assessment scores were significantly lower when compared to the control population. Moreover, a bivariate and multivariate linear regression analysis revealed that nutritional assessments and body composition were significantly correlated with biochemical markers, indicating that the nutritional status was heavily associated with systemic inflammation in patients with CRE.
In patients with CRE, the extent of malnutrition depends on the degree of tissue damage together with the site of injury . According to our experiences, perioperative nutrition support should be considered and laparoscopic surgery is superior to open surgery for treatment of radiation enteritis-induced intestinal stenosis [15, 16]. Resection or bypass of the affected bowel may ultimately lead to short bowel syndrome, which further compromises the nutritional status of patients .
Although malnutrition is acknowledged as a common concomitant disease under many circumstances, there is still a lack of universal definition and method for nutritional assessment. NRS-2002, PG-SGA and CONUT are all validated approaches that are successfully applied as nutritional assessment tools in the clinical setting [10–12]. The present study revealed that most patients with CRE suffered from malnutrition. The patients were enduring chronic malnutrition with substantial weight loss in the preceding months or year and partially food-intolerant even when severe complications occurred. With respect to biochemical markers, it is important to be aware that albumin is insufficient to assess nutritional status alone and its reduced synthesis is more related with inflammation . NLR, a simple and useful method, has emerged to represent subclinical low-grade inflammation under many circumstances [19, 20]. Hypercholesterolemia was also reported to be caused by inflammation in older patients . With respect to the relationship between nutritional assessments and biochemical markers, the present study demonstrated that serum albumin level was strongly correlated with NRS-2002, PG-SGA and CONUT scores, while others were not. This could be in a large part due to serum albumin is a more sensitive marker, reflecting both chronic malnutrition and inflammation in CRE patients.
Body composition has always been an important index of nutritional status. The traditional parameters for assessing body composition include body weight, muscular measurement, triceps skinfold-thickness and BMI . In recent years, several methods such as imaging techniques CT and MRI have been applied for body composition analysis. However, their high cost and inconvenience for bedridden patients limit their utility in the clinical practice. Dural-energy X-ray absorptiometry (DXA) is another method which can directly assess body composition but it is also restricted for its radiation exposure and inaccessibility. Bioelectrical impedance analysis (BIA), however, is a non-invasive, cheap and convenient method to indirectly evaluate body composition of patients and is widely endorsed by doctors and patients in clinical practice [23, 24]. To date, plenty of studies have validated the method of BIA in various clinical populations. Based on the spectrum of frequencies, BIA can be divided into three approaches as single-frequency BIA (SF-BIA), multi-frequency BIA (MF-BIA) and BIS. Selectively, BIS is a solid field method superior to other approaches as it measures impedance over the entire spectrum of frequencies, rather than being limited to only 1 (in the case of SF-BIA) or 2 (in MF-BIA) frequencies .
The main components of body composition are FFM and FM. It was noted that the disease could cause both lean body weight and fat tissue loss in the present study, which was also verified in other chronic diseases such as chronic obstructive pulmonary disease . BCM refers to intracellular compartments, which can be approximately speculated by ICW. BCM is also assumed to be of clinical relevance as the total mass of metabolically active, functioning cells and deemed a key parameter for body composition analysis . The present study also demonstrated that patients with CRE had lower values of ICW and TBW but a slightly higher value of ECW than those in the control group. It was speculated that capillary-interstitial and protein leak caused by chronic inflammation occurred in patients with CRE. Mineral and protein were often underappreciated for nutritional assessment. Negative protein balance occurs commonly in the context of malnutrition. Essential minerals such as calcium, iron, cooper and zinc are all indispensable for cell signaling or electron transport and lack of them may lead to devastating consequences . The present study indicated that values of BCM, ICW and FM were statistically correlated with all biochemical markers. Further analysis revealed that values of ICW, ECW, protein and CRP may explain the significant difference of BCM between CRE patients and the control populations. Thus, it could be seen that fluid and fat distribution, in particular, were the most important risk factors in CRE patients. Moreover, correlation between CRP level and body composition indicated that inflammation may be a powerful predictor of malnutrition in CRE patients.
This is the first study to evaluate the correlation between the nutritional status and systemic inflammation in patients with CRE. Although some inspiring results were obtained, there were some notified limitations. Firstly, BIA was only implemented at one time point before nutritional intervention was initiated and should be implemented at multiple time points to provide thorough tutorial suggestions for clinicians. Secondly, possible selection bias of the convenience subjects was introduced by excluding patients those who couldn’t complete these methods or questionnaire.
It is recommended that patients with CRE should undergo different nutritional assessments, including BIS combined with nutritional assessments and biochemical markers. Accordingly, appropriate strategies for nutritional support could be designed before implementing any interventions. Afterwards, re-evaluation of the nutritional and inflammatory status after a period of nutritional intervention and adjustments of further improved nutrition managements are necessary.
The present study showed that most patients with CRE suffered from malnutrition. It was intuitive to notice that these patients were thinner and had significantly lower body composition parameters compared with controls. It is concluded that nutritional assessments combined with BIS and biochemical markers are appropriate methods to assess the nutritional and inflammatory status in patients with CRE. Moreover, this study revealed that the nutritional status was significantly associated with systemic inflammation. Further researches regarding correlations of the nutritional status with complications and mortality need to be investigated in a prospective study.
BCM, Body cell mass; BIA, Bioelectrical impedance analysis; BIS, Bioelectrical impedance spectroscopy; CONUT, Controlling Nutritional Status; CRE, Chronic radiation enteritis; CRP, C-reactive protein; ECW, Extracellular water; FFM, Fat-free mass; FM, Fat mass; ICW, Intracellular water; NLR, Neutrophil/lymphocyte ratio; NRS-2002, Nutritional Risk Screening-2002; PG-SGA, Patient-Generated Subjective Global Assessment; TBW, Total body water; TC, Total cholesterol
We would like to express my gratitude to all those who participated in this study and helped me during the preparation of this paper at Department of Surgery, Jinling hospital.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Harb AH, Abou Fadel C, Sharara AI. Radiation enteritis. Curr Gastroenterol Rep. 2014;16:383.View ArticlePubMedGoogle Scholar
- Theis VS, Sripadam R, Ramani V, Lal S. Chronic radiation enteritis. Clin Oncol (R Coll Radiol). 2010;22:70–83.View ArticleGoogle Scholar
- Webb GJ, Brooke R, De Silva AN. Chronic radiation enteritis and malnutrition. J Dig Dis. 2013;14:350–7.View ArticlePubMedGoogle Scholar
- Mueller C, Compher C, Ellen DM. American society for P, enteral nutrition board of D. A.S.P.E.N. Clinical guidelines: nutrition screening, assessment, and intervention in adults. JPEN J Parenter Enteral Nutr. 2011;35:16–24.View ArticlePubMedGoogle Scholar
- Jensen GL, Mirtallo J, Compher C, Dhaliwal R, Forbes A, Grijalba RF, et al. Adult starvation and disease-related malnutrition: a proposal for etiology-based diagnosis in the clinical practice setting from the International Consensus Guideline Committee. JPEN J Parenter Enteral Nutr. 2010;34:156–9.View ArticlePubMedGoogle Scholar
- Laviano A, Koverech A, Mari A. Cachexia: clinical features when inflammation drives malnutrition. Proc Nutr Soc. 2015;74:348–54.View ArticlePubMedGoogle Scholar
- Jensen GL. Inflammation as the key interface of the medical and nutrition universes: a provocative examination of the future of clinical nutrition and medicine. JPEN J Parenter Enteral Nutr. 2006;30:453–63.View ArticlePubMedGoogle Scholar
- Nakagomi A, Kohashi K, Morisawa T, Kosugi M, Endoh I, Kusama Y, et al. Nutritional status is associated with inflammation and predicts a poor outcome in patients with chronic heart failure. J Atheroscler Thromb. 2016 [Epub ahead of print].Google Scholar
- Henson CC, Davidson SE, Lalji A, Symonds RP, Swindell R, Andreyev HJ. Gastrointestinal symptoms after pelvic radiotherapy: a national survey of gastroenterologists. Support Care Cancer. 2012;20:2129–39.View ArticlePubMedGoogle Scholar
- Algin O, Turkbey B, Ozmen E, Algin E. Magnetic resonance enterography findings of chronic radiation enteritis. Cancer Imaging. 2011;11:189–94.PubMedPubMed CentralGoogle Scholar
- Kondrup J, Allison SP, Elia M, Vellas B, Plauth M. Educational, et al. ESPEN guidelines for nutrition screening 2002. Clin Nutr. 2003;22:415–21.View ArticlePubMedGoogle Scholar
- Bauer J, Capra S, Ferguson M. Use of the scored Patient-Generated Subjective Global Assessment (PG-SGA) as a nutrition assessment tool in patients with cancer. Eur J Clin Nutr. 2002;56:779–85.View ArticlePubMedGoogle Scholar
- Ignacio De Ulibarri J, Gonzalez-Madrono A, De Villar NG, Gonzalez P, Gonzalez B, Mancha A, et al. CONUT: a tool for controlling nutritional status. First validation in a hospital population. Nutr Hosp. 2005;20:38–45.PubMedGoogle Scholar
- Stacey R, Green JT. Radiation-induced small bowel disease: latest developments and clinical guidance. Ther Adv Chronic Dis. 2014;5:15–29.View ArticlePubMedPubMed CentralGoogle Scholar
- Wang J, Yao D, Zhang S, Mao Q, Li Y, Li J. Laparoscopic surgery for radiation enteritis. J Surg Res. 2015;194:415–9.View ArticlePubMedGoogle Scholar
- Yao D, Zheng L, Wang J, Guo M, Yin J, Li Y. Perioperative alanyl-glutamine-supplemented parenteral nutrition in chronic radiation enteritis patients with surgical intestinal obstruction: a prospective, randomized, controlled Study. Nutr Clin Pract. 2015 [Epub ahead of print].Google Scholar
- Boland E, Thompson J, Rochling F, Sudan D. A 25-year experience with postresection short-bowel syndrome secondary to radiation therapy. Am J Surg. 2010;200:690–3.View ArticlePubMedGoogle Scholar
- de Mutsert R, Grootendorst DC, Indemans F, Boeschoten EW, Krediet RT, Dekker FW, et al. Association between serum albumin and mortality in dialysis patients is partly explained by inflammation, and not by malnutrition. J Ren Nutr. 2009;19:127–35.View ArticlePubMedGoogle Scholar
- Tasoglu O, Boluk H, Sahin Onat S, Tasoglu I, Ozgirgin N. Is blood neutrophil-lymphocyte ratio an independent predictor of knee osteoarthritis severity? clin rheumatol. 2016 [Epub ahead of print].Google Scholar
- Uslu AU, Deveci K, Korkmaz S, et al. Is neutrophil/lymphocyte ratio associated with subclinical inflammation and amyloidosis in patients with familial Mediterranean fever? Biomed Res Int. 2013;2013:185317.View ArticlePubMedPubMed CentralGoogle Scholar
- Ettinger Jr WH, Harris T, Verdery RB, Tracy R, Kouba E. Evidence for inflammation as a cause of hypocholesterolemia in older people. J Am Geriatr Soc. 1995;43:264–6.View ArticlePubMedGoogle Scholar
- Earthman CP. Body composition tools for assessment of adult malnutrition at the bedside: a tutorial on research considerations and clinical applications. JPEN J Parenter Enteral Nutr. 2015;39:787–822.View ArticlePubMedGoogle Scholar
- Pietrobelli A, Tato L. Body composition measurements: from the past to the future. Acta Paediatr Suppl. 2005;94:8–13.View ArticlePubMedGoogle Scholar
- Thibault R, Genton L, Pichard C. Body composition: why, when and for who? Clin Nutr. 2012;31:435–47.View ArticlePubMedGoogle Scholar
- Earthman C, Traughber D, Dobratz J, Howell W. Bioimpedance spectroscopy for clinical assessment of fluid distribution and body cell mass. Nutr Clin Pract. 2007;22:389–405.View ArticlePubMedGoogle Scholar
- Gologanu D, Ionita D, Gartonea T, Stanescu C, Bogdan MA. Body composition in patients with chronic obstructive pulmonary disease. Maedica (Buchar). 2014;9:25–32.Google Scholar
- Gletsu-Miller N, Wright BN. Mineral malnutrition following bariatric surgery. Adv Nutr. 2013;4:506–17.View ArticlePubMedPubMed CentralGoogle Scholar