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Table 4 The percentage of interfering epimer (E) and isobars (I) in serum.

From: Misleading measures in Vitamin D analysis: A novel LC-MS/MS assay to account for epimers and isobars

Volunteers 25OHD E I 25OHD+E+I Total E+I % E+I
  ng/mL ng/mL % ng/mL % ng/mL ng/mL of total
1 9.8 1.8 11.8 3.6 23.7 15.2 5.4 35.5
2 16.5 0.5 2.6 2.3 11.9 19.3 2.8 14.5
3 6.7 2.5 16.7 5.8 38.7 15.0 8.3 55.3
4 17.7 1 4.7 2.5 11.8 21.2 3.5 16.5
5 9 0 0.0 0 0.0 9.0 0 0.0
  1. E = 3-epi-25OHD3, I = 7αC4 and1αOHD3