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Table 4 The percentage of interfering epimer (E) and isobars (I) in serum.

From: Misleading measures in Vitamin D analysis: A novel LC-MS/MS assay to account for epimers and isobars

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25OHD

E

I

25OHD+E+I

Total E+I

% E+I

 

ng/mL

ng/mL

%

ng/mL

%

ng/mL

ng/mL

of total

1

9.8

1.8

11.8

3.6

23.7

15.2

5.4

35.5

2

16.5

0.5

2.6

2.3

11.9

19.3

2.8

14.5

3

6.7

2.5

16.7

5.8

38.7

15.0

8.3

55.3

4

17.7

1

4.7

2.5

11.8

21.2

3.5

16.5

5

9

0

0.0

0

0.0

9.0

0

0.0

  1. E = 3-epi-25OHD3, I = 7αC4 and1αOHD3