Fig. 5

a Neurosphere exhibited adherent growth after several hours of incubation. Extensive thin, elongated projections were observed, connecting adjacent neurospheres into a complex network (100x); b Immunocytochemical staining showing primary NSCs with Nestin-positive cytoplasm and Nestin-negative nuclei (200x); c Small, round/oval/triangle NSE-positive neurons with thin and long projections were observed at day 5 since the differentiation induction (200x). Synaptic connections have been established between neurons (red arrow); and d large, irregular-shaped GFAP-positive astrocytes with multiple short and flat projections were observed at day 5 since the differentiation induction (200x)