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Table 1 Composition of experimental diets

From: Polyphenolic compounds appear to limit the nutritional benefit of biofortified higher iron black bean (Phaseolus vulgarisL.)

Ingredient

Standard bean diet

Biofortified bean diet

 

g/kg (by formulation)

Biofortified Beans (88 μg Fe/g)

_

400

Standard Beans (59 μg Fe/g)

400

_

Corn

350

350

Corn oil

30

30

Dry skim milk

100

100

Corn starch

46.75

46.75

Vitamin/mineral premix (no Fe) 1

70

70

DL-Methionine

2.5

2.5

Choline Chloride

0.75

0.75

Total (g)

1000

1000

Selected components

mean ± SEM, n = 5 (by analysis)

Fe (μg Fe/g) 2

39.4 ± 0.2b

52.9 ± 0.9a

Phytate:Fe molar ratio3

8.25 ± 0. 65a

8.95 ± 0.72a

  1. 1Vitamin and mineral premix provided/kg diet (330002 Chick vitamin mixture; 235001 Salt mix for chick diet; Dyets Inc. Bethlehem, PA).
  2. 2Iron concentrations in the diets were determined by an inductively-coupled argon-plasma/atomic emission spectrophotometer (ICAP 61E Thermal Jarrell Ash Trace Analyzer, Jarrell Ash Co. Franklin, MA) following wet ashing.
  3. 3Method for determining phytate contents are described in the materials and methods section.
  4. a,bWithin a row, means without a common letter are different, P < 0.05.