PI3-K/Akt siganling regulates FN-expression and does not signal downstream of GLN-mediated FN-Integrin osmosignaling after HS. A) IEC-6 cells were treated for 1 h with either media, GRGDSP (50 μM), or GRGESP (50 μM), prior to GLN treatment (0 mM or 10 mM) under basal (37°C) and stressed conditions (43°C). Procaspase-3, cleaved caspase-3 levels and ß-actin were measured via Western blot and cleaved Caspase-3 levels are presented as fold change ± SEM (n = 3). B) IEC-6 cells were treated as described in Figure 2A. Representative Western blots of 3 independent experiments of [S(P)473]Akt and total Akt are shown as mean fold change relative to total Akt ± SEM and normalized to 0 mM GLN. C) FN levels with/without GLN (10 mM) and LY294002 (25 μM) treatment under unstressed (37°C) or stressed conditions (43°C) were determined by Western blot after 3 h recovery. Densitometric analysis of FN expression as mean fold change relative to 0 mM GLN cells ± SEM (n = 3-8).