A randomised, double-blind, placebo controlled cross-over study to determine the gastrointestinal effects of consumption of arabinoxylan-oligosaccharides enriched bread in healthy volunteers

Table 2 Hybridisation conditions for oligonucleotide probes used in this study

Probe name	Sequence (5’ to 3’)	Hybridisation pre-treatment	Formamide (%) for hybridisation	Temperature (°C)		Bacteria covered
Probe name	Sequence (5’ to 3’)	Hybridisation pre-treatment	Formamide (%) for hybridisation	Hybridisation Washing		Bacteria covered
Ato291	GGTCGGTCTCTCAACCC	Lysozyme	0	50	50	Atopobuim- Coriobacterium	[33]
Bac303	CCAATGTGGGGGACCTT	None	0	46	48	Bacteroides – Prevotella	[34]
Bif164	CATCCGGCATTACCACCC	Lysozyme	0	50	50	Bifidobacterium spp	[35]
Chis150	TTATGCGGTATTAATCTYCCTTT	None	0	50	50	Clostridium histolyticum- Clostridium perfringens	[36]
Erec482	GCTTCTTAGTCARGTACCG	None	0	50	50	Eubacterium rectale- Clostridium coccoides	[36]
Lab158	GGTATTAGCAYCTGTTTCCA	Lysozyme	0	50	50	Lactobacillus- Enterococcus	[37]
Eco1531	CACCGTAGTGCCTCGTCATCA	None	35	37	37	Escherichia coli	[38]
Rrec584	TCAGACTTGCCGYACCGC	None	0	50	50	Roseburia and Eubacterium rectale	[39]
Fprau645	CCTCTGCACTACTCAAGAAAAAC	Lysozyme	15	46	48	Faecalibacterium prausnitzzi	[40]
EUB338‡	GCTGCCTCCCGTAGGAGT	None	35	46	48	Total bacteria 1	[41]
EUB338II‡	GCAGCCACCCGTAGGTGT	None	35	46	48	Total bacteria	[41]
EUB338III‡	GCTGCCACCCGTAGGTGT	None	35	46	48	Total bacteria	[41]

ISSN: 1475-2891