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Fig. 4 | Nutrition Journal

Fig. 4

From: Masquelier’s grape seed extract: from basic flavonoid research to a well-characterized food supplement with health benefits

Fig. 4

Representative chromatogram of Masquelier’s grape seed extract measured by high performance liquid chromatography (HPLC) using a C18 base-deactivated reversed-phase column (e.g. ProntoSIL 120-5-C18H, Bischoff Chromatography, Leonberg, Germany) with a gradient elution system of water/trifluoroacetic acid (TFA) 0.005% (v/v) and acetonitrile 65% (v/v)/TFA 0.005% (v/v) at a flow rate of 0.7 ml/min, at ambient column temperature and with UV detection at a wavelength of λ = 280 nm. The peaks in the chromatogram have been identified as the following individual monomeric and oligomeric flavan-3-ols: 1 = procyanidin B1, 2 = procyanidin B3, 3 = (+)-catechin, 4 = dimeric procyanidin consisting of (+)-catechin-(-)-epicatechin, 5 = procyanidin B4, 6 = procyanidin B2, 7 = (-)-epicatechin, 8 = prodcyanidin B2 gallate. The data were provided by the extract’s manufacturer

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