Fig. 3

NSCs derived from neural tube were cultured in serum-free complete medium. a. In the beginning of culture, cells were small, round and translucent (100x). b: After 12 h, cells grew into small, round cell mass (200×). c: After 24 h, cell mass grew bigger and cell debris was observed (100×). d: On day 5, round cell mass grew into big neurosphere with cells well organized (200×)