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Table 2 Comparison of HPLC methods for total plasma vitamin C analysis

From: A simple method for plasma total vitamin C analysis suitable for routine clinical laboratory use

 

ECa

Other UV methodsb

Present UV method

Equipment

 Availability

Limited

Wide

Wide

 Cost

High

Moderate

Moderate

 Maintenance

Complex, lengthy

Simple

Simple

Separation column

Dedicated ion-pairing

Reverse phase or ion-pairing

Reverse phase

Number of reagents

14

3-6

4

Reagent preparation time (h)

4

1.5

1

Number of proceduresc

7

4-6

4

Number of pH adjustments

3

0-2

0

Time prior to sample injection (h)d

4-5

2-3

2

Chromatographic run time (min)

8

2-16

6

pH

 Mobile phase

4.8

4.5-5.7

2.7

 Low pH throughout procedure

No

No

Yes

  1. aAs described in the Methods section
  2. bAs described in [59, 63, 64]
  3. cPreparation of reagents and mobile phase, pH adjustments and sample handling
  4. dPreparation of mobile phase, system equilibration, reduction reaction and standards and control analysis