From: A simple method for plasma total vitamin C analysis suitable for routine clinical laboratory use
 | ECa | Other UV methodsb | Present UV method |
---|---|---|---|
Equipment | |||
 Availability | Limited | Wide | Wide |
 Cost | High | Moderate | Moderate |
 Maintenance | Complex, lengthy | Simple | Simple |
Separation column | Dedicated ion-pairing | Reverse phase or ion-pairing | Reverse phase |
Number of reagents | 14 | 3-6 | 4 |
Reagent preparation time (h) | 4 | 1.5 | 1 |
Number of proceduresc | 7 | 4-6 | 4 |
Number of pH adjustments | 3 | 0-2 | 0 |
Time prior to sample injection (h)d | 4-5 | 2-3 | 2 |
Chromatographic run time (min) | 8 | 2-16 | 6 |
pH | |||
 Mobile phase | 4.8 | 4.5-5.7 | 2.7 |
 Low pH throughout procedure | No | No | Yes |