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Table 4 Summary of studies comparing tracer methods to estimate fractional calcium absorptiona

From: Can serum isotope levels accurately measure intestinal calcium absorption compared to gold-standard methods?

Subjects

Measures, New Method, Gold Standard Method (GSM)

Linear Regression Model to Estimate FCAb

Variance, p-value

265 women

554 measures, 5-h serum, GSM: average of oral to iv tracer valuesin multiple serum and urine samples collected for 6-10 days

cFCA = 2.3045(Serum) + 0.021953(Weight0.425) (Height0.725) - 0.1735

R2 0.91

≥35 years old [3]

p not provided

30 men

New method at baseline and GSM 6 days later, 5-h serum, GSM: quotient of 5-h oral radioactivity to 3-h iv radioactivity

cFCA = (Serum)0.92373 x (0.385 x (Height0.5285) (Weight0.3721))

R2 0.90

20-60 years old [20]

p < 0.001

12 men

24 measures, 5-h serum, GSM: average of oral to iv tracer values in multiple serum and urine samples collected at multiple times, and quotient of 5-h oral radioactivity to 3-h iv radioactivity

Not provided

R2 0.92

36 ± 5 years old [21]

p not provided

19 men and women

19 measures, 4-h serum, GSM: ratio of cumulative recovery of oral to iv tracers in 24-h urine collection

eFCA = ratio of oral calcium tracer to intravenous tracer

R2 0.83

≥50 years old [10]

p < 0.001

22 girls

31 measures, 4-h serum, GSM: Average of 3 values- oral to iv ratio in 24-h serum, 24-h urine and kinetic model

c,d,fFCA = 1.334(Serum)0.7872BSA1.7132e(-0.01652PMA)

R2 0.94

10-15 years old [8]

p < 0.001

26 women

129 measures, 4, 5, 6 h serum, GSM: Average of 3 values- oral to iv ratio in 24-h serum, 24-h urine and kinetic model

cFCA = 1.3609(4 h Serum)0.8703BSA0.8708

R2 0.78

19-67 years old [9]

p < 0.001

cFCA = 1.4065(5 h Serum)0.8437BSA0.7785

R2 0.78

p < 0.001

cFCA = 1.5828(6 h Serum)0.8636BSA0.7142

R2 0.81

p < 0.001

  1. aStudies summarized in this table reported the amount of variance (R2) in fractional calcium absorption explained by a spot serum tracer level. Two other notable studies [12, 17] reported correlation coefficients only
  2. b“FCA” denotes fractional calcium absorption
  3. cIn this formula, the serum level reflects the radioactivity or stable calcium isotope level as a fractional dose per mL, per gram/mL of serum calcium
  4. d“BSA” refers to body surface area as defined by the DuBois equation [22]
  5. eIn this study, the oral tracer was given with breakfast and the intravenous tracer was given two hours later, with serum drawn 4 h after breakfast
  6. f“PMA” refers to post-menarchal age