Table 1 Primer sequences for quantitative real-time RT-PCR
Gene | Forward primer (F) | Reverse primer (R) |
|---|---|---|
GADPH | TCCCTGAGCTGAACGGGAAG | GGAGGAGTGGGTGTCGCTGT |
Kras | TAGTTGGAGCTGGTGGCGTA | CCTCTTGACCTGCTGTGTCG |
Erk | ACAGGCTGTTCCCAAATGCT | CGAACTTGAATGGTGCTTCG |
Akt | GTCGCCTGCCCTTCTACAAC | CACACGATACCGGCAAAGAA |
Bcl-xl | GCATTGTGGCCTTTTTCTCC | GCTGCTGCATTGTTCCCATA |
Caspase 9 | GAGGGAAGCCCAAGCTCTTT | CACTGGGTGTGGGCAAACTA |
P65 | CCTGGGAATCCAGTGTGTGA | GCACTGTCACCTGGAAGCAG |
IκBα | GAAGTGATCCGCCAGGTGAA | CTCACAGGCAAGGTGTAGGG |