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Table 2 Average DNA yield obtained using the four commercial DNA extraction kits1.

From: Comparison of DNA extraction kits for PCR-DGGE analysis of human intestinal microbial communities from fecal specimens

DNA extraction kit1

DNA yield (mg DNA/g dry wt feces)3

M

52.4 ± 14.5b

Q

57.0 ± 22.6b

FSp

151.3 ± 47.1a

FSo

187.2 ± 69.4a

  1. The following extractions accounted for various fecal specimen weights (10, 25, 50, 100, and 200 mg) and DNA yield was normalized by percent fecal dry matter (26%, 35%, and 41%)2.
  2. 1M, Mobio Ultra Clean® Fecal DNA Isolation Kit; Q, QIAamp® DNA Stool Mini Kit; FSp, FastDNA® SPIN Kit; FSo, FastDNA® SPIN Kit for Soil
  3. 2Values of DNA yield were based on n = 45/DNA extraction method and were normalized based on the dry weight of the respective fecal sample
  4. 3Treatment groups with different letters indicate significant differences between groups (P < 0.05). Values are means ± SE.
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Nutrition Journal

ISSN: 1475-2891

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