Table 7 Gradient of mobile phases used for HPLC analysis of propolis.
time (min) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
mobile phase | 0 | 15 | 25 | 30 | 40 | 50 | 60 | 75 | 80 |
%A | 90 | 50 | 50 | 40 | 40 | 10 | 10 | 90 | 90 |
%B | 10 | 50 | 50 | 60 | 60 | 90 | 90 | 10 | 10 |
time (min) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
mobile phase | 0 | 15 | 25 | 30 | 40 | 50 | 60 | 75 | 80 |
%A | 90 | 50 | 50 | 40 | 40 | 10 | 10 | 90 | 90 |
%B | 10 | 50 | 50 | 60 | 60 | 90 | 90 | 10 | 10 |