Table 2 The mobile phases studied.
No | Solvents | Proportions (v/v) |
|---|---|---|
1 | toluene – ethyl acetate – formic acid (98–100%) | 36 : 12 : 5 |
2 | cyclohexane – ethyl acetate – formic acid (98–100%) | 30 : 15 : 5 |
3 | toluene – ethyl acetate – glacial acetic acid | 36 : 12 : 5 |
4 | cyclohexane – ethyl acetate – glacial acetic acid | 31 : 14 : 5 |
5 | n-hexane – ethyl acetate – formic acid (98–100%) | 31 : 14 : 5 |
6 | toluene – acetone – formic acid (98–100%) | 38 : 10 : 5 |
7 | n-hexane-ethyl acetate – glacial acetic acid | 31 : 14 : 5 |
8 | petroleum ether (40–70°C) – ethyl acetate – formic acid (98–100%) | 30 : 15 : 5 |
9 | carbon tetrachloride – acetone – formic acid (98–100%) | 35 : 10 : 5 |
10 | n-hexane – ethyl acetate – glacial acetic acid | 30 : 20 : 1.5 |
11 | chloroform – methanol – formic acid (98–100%) | 44 : 3.5 : 2. 5 |