Table 1 Micronutrient assay methods.
Analyte | Method | Ref. |
|---|---|---|
RBC folate (nmol/L) | Quantaphase Folate Radioassay Kit, Bio-Rad Laboratories | 7 |
Retinol (μg/dL) | Isocratic reversed-phase HPLC with UV/Vis detection | 8 |
Ascorbic acid (mg/dL) | Isocratic reversed-phase HPLC with Electrochemical detection* | 7 |
Alpha tocopherol (mg/dL) | Isocratic reversed-phase HPLC with UV/Vis detection | 8 |
Alpha carotene (μg/dL) | Isocratic reversed-phase HPLC with UV/Vis detection | 8 |
Beta carotene (μg/dL) | Isocratic reversed-phase HPLC with UV/Vis detection | 8 |
Ferritin (μg/L) | Quantlmune Ferritin IRMA kit, Bio-Rad Laboratories | 7 |
25-hydroxyvitamin D (nmol/L) | Quantlmune Ferritin IRMA kit, Bio-Rad Laboratories | 7 |
Homocysteine (μmol/L) | Isocratic reversed-phase HPLC with Fluorescence detection | 9 |
C-reactive protein (mg/L) | Latex-enhanced nephelometry, Behring Nephelometer Analyzer for NHANES assays and Hitachi 917 Analyzer for Multiple Supp user assays | 7 |
Total cholesterol (mg/dL) | Timed endpoint, coupled enzymatic methodology | 10 |
LDL-cholesterol (mg/dL) | Calculated** | 7 |
HDL-cholesterol (mg/dL) | Polyanion/divalent cation precipitation of other lipoproteins followed by timed endpoint, coupled enzymatic methodology | 10 |
Triglycerides (mg/dL) | Timed endpoint, coupled enzymatic methodology | 11 |