Figure 3

Confocal microscopy of polarized Caco-2 cells treated with MnGluc or MnOxP- analysis of MT fluorescence. Quantification and distribution of MT in response to 12.5 μM MnGluc or MnOxP are shown. Root Mean Square fluorescence intensity quantification from a representative long experiment is in panel A. Symbols (o, #, *, §) indicate significant differences (p < 0.05) between Caco-2 cells responses. Panel B shows confocal MT images from the same samples at 30 min and 24 h, in agreement with data in panel A and displaying that the regular mitochondria distribution is preserved in treated cells. Transversal 2D free projection max (panel C) from a brief experiment displays both AO and MT signals in the presence of MnOxP. MT shows a transient redistribution of mitochondria close to the apex of cells at 15 min. AO helps in localize the nuclei respect to MT and demonstrates the cells viability.