Figure 3

(A) Effect of vitamin A supplementation on hepatic C/EBPα mRNA and protein levels in WNIN/Ob lean and obese rats. Quantified densitometric values are expressed relative of 1 for lean control rats (AI). (B) Representative photo of C/EBPα PCR product, stained by ethidium bromoide. (C) Representative photo of β-Actin PCR product (internal control), stained by ethidium bromide. (D) Quantified densitometric values from the western blot are expressed relative of 1 for A-I (lean) as control. (E) Representative Western blot of hepatic C/EBPα protein. (F) Ponseau stained western blot. Values are means ± SE for 3-4 rats at P ≤0.05 level. Comparisons were made between normal and high vitamin A-fed groups of each phenotype (AI-Lean: 2.6 mg vitamin A/kg diet, AII-Lean: 129 mg vitamin A/kg diet, BI-Obese: 2.6 mg vitamin A/kg diet and BII-Obese: 129 mg/kg diet).