Figure 1

Specificity of the vitamin B ₁ and B ₆ assay. Chromatograms of PN placebo (A and D), spiked with the B₁ and B₆ (B and E) and PN containing the multivitamin (C and F) obtained by HPLC at wavelength of 250 and 295 nm, respectively, using column of reverse phase C₁₈ and a mixture of methanol:water (27:73, v/v) and sodium hexano sulfonate 1,4%, pH 3.0 as mobile phase (n = 3).