Table 2 Retention times, MRM transitions and ESI conditions of analytes.
From: Misleading measures in Vitamin D analysis: A novel LC-MS/MS assay to account for epimers and isobars
Analytes | Retention time (min) | Transition (m/z) Precursor→ Product | Collision energy (eV) |
|---|---|---|---|
|  |  | 401.3 → 383.1 | 36 |
25OHD3 | 13.5 | 401.3 → 365.1 | 41 |
|  |  | 401.3 → 159.2 | 46 |
|  |  | 413.3 → 395.5 | 46 |
25OHD2 | 11.1 | 413.3 → 377.2 | 51 |
|  |  | 413.3 → 355.5 | 53 |
|  |  | 401.3 → 383.1 | 36 |
3-epi-25OHD3 | 13.1 | 401.3 → 365.1 | 41 |
|  |  | 401.3 → 159.2 | 46 |
|  |  | 401.3 → 383.1 | 36 |
1αOHD3 | 11.1 | 401.3 → 365.1 | 41 |
|  |  | 401.3 → 159.2 | 46 |
|  |  | 401.3 → 383.1 | 36 |
7αC4 | 10.5 | 401.3 → 365.1 | 41 |
|  |  | 401.3 → 159.2 | 46 |
Stanozolol-D3 (I.S) | 3.09 | 332.2 → 81.2 | 42 |