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Table 2 Retention times, MRM transitions and ESI conditions of analytes.

From: Misleading measures in Vitamin D analysis: A novel LC-MS/MS assay to account for epimers and isobars

Analytes Retention time (min) Transition (m/z) Precursor→ Product Collision energy (eV)
   401.3 → 383.1 36
25OHD3 13.5 401.3 → 365.1 41
   401.3 → 159.2 46
   413.3 → 395.5 46
25OHD2 11.1 413.3 → 377.2 51
   413.3 → 355.5 53
   401.3 → 383.1 36
3-epi-25OHD3 13.1 401.3 → 365.1 41
   401.3 → 159.2 46
   401.3 → 383.1 36
1αOHD3 11.1 401.3 → 365.1 41
   401.3 → 159.2 46
   401.3 → 383.1 36
7αC4 10.5 401.3 → 365.1 41
   401.3 → 159.2 46
Stanozolol-D3 (I.S) 3.09 332.2 → 81.2 42