Table 1 Summary of assay validation results.
From: Misleading measures in Vitamin D analysis: A novel LC-MS/MS assay to account for epimers and isobars
Compounds | Linear range (ng/mL) | LLOD (ng/mL) | Recovery (%) at 50 ng/mL | Concentration (ng/mL) | r2 | Intraday (%CV) | Interday (%CV) | Accuracy (%) |
|---|---|---|---|---|---|---|---|---|
25OHD3 | 0.5-84.4 | 0.25 | 95 | 12.5 | 0.9999 | 2.1 | 12.1 | 97.5 |
| Â | Â | Â | Â | 25 | Â | 4.6 | 6.7 | 92.9 |
| Â | Â | Â | Â | 50 | Â | 7.1 | 4.1 | 92.2 |
25OHD2 | 0.5-84.4 | 0.25 | 88 | 12.5 | 0.9996 | 7.5 | 8.1 | 99.4 |
| Â | Â | Â | Â | 25 | Â | 9.1 | 8.6 | 97.5 |
| Â | Â | Â | Â | 50 | Â | 6.8 | 5.5 | 101.2 |