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Table 1 Summary of assay validation results.

From: Misleading measures in Vitamin D analysis: A novel LC-MS/MS assay to account for epimers and isobars

Compounds Linear range (ng/mL) LLOD (ng/mL) Recovery (%) at 50 ng/mL Concentration (ng/mL) r2 Intraday (%CV) Interday (%CV) Accuracy (%)
25OHD3 0.5-84.4 0.25 95 12.5 0.9999 2.1 12.1 97.5
     25   4.6 6.7 92.9
     50   7.1 4.1 92.2
25OHD2 0.5-84.4 0.25 88 12.5 0.9996 7.5 8.1 99.4
     25   9.1 8.6 97.5
     50   6.8 5.5 101.2