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Table 1 Composition of experimental diets

From: Biofortified red mottled beans (Phaseolus vulgaris L.) in a maize and bean diet provide more bioavailable iron than standard red mottled beans: Studies in poultry (Gallus gallus) and an in vitro digestion/Caco-2 model

Ingredient Low-Fe Bean Diet High-Fe Bean Diet
g/kg (by formulation)
High-Fe Beans (71 μg Fe/g) _ 600
Low-Fe Beans (49 μg Fe/g) 600 _
Corn 200 200
Corn oil 30 30
Dry skim milk 100 100
Vitamin/mineral premix (no Fe) 1 70 70
DL-Methionine 2.5 2.5
Choline Chloride 0.75 0.75
Total (g) 1000 1000
Concentrations of selected components mean ± SEM, n = 5 (by analysis)4
Fe (μg Fe/g) 2 42.9 ± 1.2a 54.6 ± 0.9b
Total Phenols (gallic acid, μg/g) 3 103.5 ± 5.5a 101.8 ± 6.1a
Phytate:Fe molar ratio 3 8.28 ± 9.2a 8.59 ± 1.06a
  1. 1Vitamin and mineral premix provided/kg diet (330002 Chick vitamin mixture; 230000 Salt mix for chick diet; Dyets Inc. Bethlehem, PA).
  2. 2Iron concentrations in the diets were determined by an inductively-coupled argon-plasma/atomic emission spectrophotometer (ICAP 61E Thermal Jarrell Ash Trace Analyzer, Jarrell Ash Co. Franklin, MA) following wet ashing.
  3. 3Method for determining phenol concentrations and phytate contents are described in the materials and methods section.
  4. 4Within a row, means without a common letter are significantly different, P < 0.05.