Pathogen detection, testing, and control in fresh broccoli sprouts
© Fahey et al; licensee BioMed Central Ltd. 2006
Received: 21 February 2006
Accepted: 21 April 2006
Published: 21 April 2006
The recent increased interest in consuming green vegetable sprouts has been tempered by the fact that fresh sprouts can in some cases be vehicles for food-borne illnesses. They must be grown according to proper conditions of sanitation and handled as a food product rather than as an agricultural commodity. When sprouts are grown in accordance with the criteria proposed from within the sprout industry, developed by regulatory agencies, and adhered to by many sprouters, green sprouts can be produced with very low risk. Contamination may occur when these guidelines are not followed.
A one year program of microbial hold-and-release testing, conducted in concert with strict seed and facility cleaning procedures by 13 U.S. broccoli sprout growers was evaluated. Microbial contamination tests were performed on 6839 drums of sprouts, equivalent to about 5 million consumer packages of fresh green sprouts.
Only 24 (0.75%) of the 3191 sprout samples gave an initial positive test for Escherichia coli O157:H7 or Salmonella spp., and when re-tested, 3 drums again tested positive. Composite testing (e.g., pooling up to 7 drums for pathogen testing) was equally sensitive to single drum testing.
By using a "test-and-re-test" protocol, growers were able to minimize crop destruction. By pooling drums for testing, they were also able to reduce testing costs which now represent a substantial portion of the costs associated with sprout growing. The test-and-hold scheme described herein allowed those few batches of contaminated sprouts to be found prior to packaging and shipping. These events were isolated, and only safe sprouts entered the food supply.
Green sprouts have been a part of the human diet for much of recorded history. Their commercial production has been a small niche industry in the U.S. for the past 30 or so years, but they are much more widely consumed in countries like Japan, where they are part of mainstream diets. In the past 8 years broccoli sprouts have gained increased scientific attention due to their high content of phytochemicals that are involved in protection against cancer and other degenerative diseases [1–7].
Industry experts estimate that about 15 million pounds of fresh green sprouts are now grown in the U.S. annually and the vast majority of these are alfalfa sprouts. Sprouts are grown from seeds placed in environmentally controlled, hydroponic conditions and incubated in warm, moist, nutrient-rich conditions [1.5 m. diameter, slowly rotating "drums" charged with ca. 20 kg of seed], which are ideal environments for microbial growth. If Escherichia coli or Salmonella spp. are present on the surface of the seed, it is likely that they will multiply in the sprouting environment. To date, no practical methods have been developed to check the growth of these contaminants during germination and sprout growth or processing. They must therefore be prevented from entering the process, or if contamination occurs, affected final product must be identified and destroyed. It is therefore essential that seeds to be used for sprouting, undergo surface-disinfection by treatment with a biocide. The efficacy of such agents, most notably calcium hypochlorite, has been extensively documented in the laboratories of Beuchat and colleagues, [8–12] as well as others [13–15]. When these agents are used correctly, the resulting sprouts are safe to eat. A recommendation to use such a surface disinfection process is now part of a guidance that the FDA issued in 1999 .
This recent increased interest in consuming green vegetable sprouts has been tempered by the fact that fresh sprouts can in some cases be vehicles for food-borne illnesses, if not grown according to proper conditions of sanitation and handled as a food product rather than as an agricultural commodity [17–19]. When sprouts are grown in accordance with the criteria proposed from within the sprout industry, developed by the FDA , and adhered to by many sprouters, green sprouts can be produced with very low risk. Contamination can occur when these guidelines are not followed [19, 20].
Whereas chlorination of seeds dramatically reduces the chances of growing a contaminated product, adherence to good manufacturing practices and integration of Hazard Analysis Critical Control Point (HACCP) plans  further reduce such chances. The ultimate control point for microbial safety is the product, thus incidents of contamination, when they occur, can only be ascertained by testing each lot of sprouts prior to releasing them for sale (hold-and-release testing). This is best done by testing the spent irrigation water. The United States FDA has issued a guidance document for growers, with considerable input from the sprouting industry, which is designed to prevent contaminated sprouts from ever reaching the public . The FDA followed up issuance of this guidance with an inspection of 150 sprouters, and determined that only about half of them were complying with the guidance . We have examined a subset of sprouters in whose facilities compliance with the FDA guidance was verified based upon inspections by two third party auditors.
The growers, 13 producers or co-packers of broccoli (BroccoSprouts®) and other sprouts for the company Brassica Protection Products (Baltimore, MD, USA) during the 2001 calendar year, were selected for this review. These growers were selected because they are established companies that grow many different kinds of green sprouts, and they had previously agreed to comply with rigid standards of sanitation. Geographically, they are distributed around the United States with respect to both plant siting and product distribution. In particular, all surveyed growers follow all of the steps specified in the FDA Guidance for Industry . Specifically, they surface-disinfect all seeds and perform microbial testing of the spent irrigation water from each batch of green sprouts produced, and they are subject to announced and unannounced third party audits and state and federal inspections of these procedures.
Seed treatment and sprout production
Briefly, surface disinfection of seeds is accomplished by exposing seeds to 20,000 ppm of calcium hypochlorite for 15 min, followed by extensive rinsing to remove residual chlorine. Sprouts are grown in trays or drums which are provided with only light (fluorescent, incandescent and/or filtered sunlight), heat (constant temperature), and clean water (filtered, well, or municipal-chlorinated). A sample of the spent irrigation water, typically 1 L, is collected after 48 hours of sprout growth. Since the sprouts typically are grown for 72 – 120 h, a rapid microbial test permits the sprouters to abort contaminated batches of sprouts prior to packaging, or to shipping and distribution. All sprouts were held until test results were obtained. In cases where presumptive positives were obtained, the refrigerated, unused portion of the 48 hour spent irrigation water sample was used for re-testing as specified by the FDA guidance . Typically, the confirmation analysis was performed using a different, more specific method. In at least one situation, the grower was collecting individual samples, and the testing lab was preparing a composite sample for testing. In this case, a confirmation was performed on each individual sample (one water sample per drum).
All growers who contributed data for this study were requested to directly forward their hold-and-release testing results for the calendar year 2001 to Quality Associates Incorporated (QAI; Columbia, MD, USA). Many growers included a description of the green sprouts grown in each batch with these results. In addition to broccoli sprouts, the growers reported growing the following green sprouts: alfalfa, clover, radish, onion, pea, sunflower, and a variety of mixes. Data that was exclusively derived from batches of bean sprouts (e.g. mungbean and soybean) were not used herein. However, in some cases pooled water samples that were tested included water from both green sprouts and bean sprouts, and these test results are included in the analyses.
The raw data utilized were copies of the analytical reports, as they were received by the growers from the contract analytical laboratories that performed the microbial testing. All growers but one used the services of an external microbiological testing laboratory, to which samples could be delivered on the day of collection. One grower had instead set up an in-house laboratory for these analyses and all laboratory notes were provided to us by this grower.
Methods used for hold-and-release testing.
Test kit name
E. coli 0157:H7
Biocontrol VIP EHEC for E.coli O157:H7
Biocontrol Assurance EIA EHEC
Neogen Reveal E.coli O157:H7
Biocontrol VIP for Salmonella
bioMerieux VIDAS SLM
Performance Tested Method
Neogen Reveal Salmonella
Biocontrol Assurance Gold EIA Salmonella
Tecra Salmonella VIA
Bacteriological Analytical Method Chapter 5 23
Distribution of number of drums composited into each sample for pathogen testing.
Whereas all growers produced broccoli sprouts, many of the composite samples taken included samples from non-broccoli sprouts (alfalfa in most cases). Presumptive detection of Salmonella spp. or E. coli O157:H7 occurred in 24 of 3191 samples (0.75%) from a total of 6839 drums. As described in the FDA Guidance , the detections were considered presumptive and in general, the manufacturers claims for sensitivity of these tests ranged from 70% to 100% and the specificity ranged from 78% to 100% (Table 1). The majority of the tests are reported to have a rate of false positive responses below 2%. In most cases of presumptive positives, the water sample was then reanalyzed. In two of these cases, a follow-up analysis was again positive for Salmonella spp., and the sprouts were destroyed. In a single case a presumptive detection of E. coli O157:H7 was assumed by the grower to be real without re-testing; the sprouts were destroyed. None of these 3 samples included broccoli sprouts. Additional prophylactic measures were taken as outlined in reference . Sprouts were held at the growing facilities until microbial testing results were confirmed. There were no instances in which contaminated sprouts were released for distribution.
Presumptive positive samples grouped by composite number of drums represented by the water sample tested.
No. of drums
No. of presumptive positives
No. of samples
Total No. of drums
Presumptive positives per drum (%)
HACCP-based microbial hold-and-release testing, conducted in concert with strict seed and facility sanitation procedures by 13 U.S. broccoli sprout growers (representing tests of 6839 drums of sprouts or about 5 million consumer packages of fresh green sprouts) has resulted in the successful identification and elimination of hazardous microbial contamination when and where it existed. Less than half a percent of the samples tested gave an initial positive test for E. coli O157:H7 or Salmonella spp. When re-tested, only 10% of these (3 drums out of 6839), were positive for the presence of these organisms. By using a "test-and-re-test" protocol, growers were able to minimize crop destruction. By pooling drums for testing, they were also able to reduce testing costs which now represent a substantial portion of the costs associated with sprout growing. The test-and-hold scheme described herein allowed those few batches of contaminated sprouts to be found prior to packaging and shipping. These events were isolated, and only safe sprouts entered the food supply.
With proper attention to growing conditions and testing procedures, the advantages of fresh green sprouts can be safely realized by those who choose to eat sprouts as part of a healthy diet.
We acknowledge many colleagues, in particular Douglas Archer, Arthur Davis, Katherine Stephenson, Paul Swidersky, and Paul Talalay, for perceptive, critical reading of early versions of this manuscript. Our special thanks go to the audited sprout companies for permitting us access to their records and for their close cooperation. Microbial testing was paid for those individual commercial sprout companies. Data collection and evaluation was paid for by Brassica Protection Products and performed by an external quality assurance testing and auditing firm (Quality Associates, Inc.), and the senior author's involvement was supported by unrestricted research funds generously provided by the Lewis B. and Dorothy Cullman Foundation.
- Fahey JW, Haristoy X, Dolan PM, Kensler TW, Scholtus I, Stephenson KW, Talalay P, Lozniewski A: Sulforaphane inhibits extracellular, intracellular, and antibiotic-resistant strains of Helicobacter pyloriand prevents benzo[a]pyrene-induced stomach tumors. Proc Natl Acad Sci USA. 2002, 99: 7610-7615. 10.1073/pnas.112203099.View ArticlePubMedPubMed CentralGoogle Scholar
- Fahey JW, Zhang Y, Talalay P: Broccoli sprouts: an exceptionally rich source of inducers of enzymes that protect against chemical carcinogens. Proc Natl Acad Sci USA. 1997, 94: 10367-10372. 10.1073/pnas.94.19.10367.View ArticlePubMedPubMed CentralGoogle Scholar
- Fahey JW, Zalcmann AT, Talalay P: The chemical diversity and distribution of glucosinolates and isothiocyanates among plants. Phytochemistry. 2001, 56 (1): 5-51. 10.1016/S0031-9422(00)00316-2. [corrigendum: Phytochemistry 59, 237.]View ArticlePubMedGoogle Scholar
- Brooks JD, Paton VG, Vidanes G: Potent induction of phase 2 enzymes in human prostate cells by sulforaphane. Cancer Epidemiol Biomarkers Prev. 2001, 10 (9): 949-954.PubMedGoogle Scholar
- Gao X, Dinkova-Kostova AT, Talalay P: Powerful and prolonged protection of human retinal pigment epithelial cells, keratinocytes, and mouse leukemia cells against oxidative damage: the indirect antioxidant effects of sulforaphane. Proc Natl Acad Sci USA. 2001, 98: 15221-15226. 10.1073/pnas.261572998.View ArticlePubMedPubMed CentralGoogle Scholar
- Shapiro TA, Fahey JW, Wade KL, Stephenson KK, Talalay P: Disposition of chemoprotective glucosinolates and isothiocyanates of broccoli sprouts. Cancer Epidemiol Biomarkers Prev. 2001, 10 (9): 501-508.PubMedGoogle Scholar
- Talalay P, Fahey JW: Phytochemicals from cruciferous plants protect against cancer by modulating carcinogen metabolism. J Nutr. 2001, 131: 3027S-3033S.PubMedGoogle Scholar
- Beuchat LR: Comparison of chemical treatments to kill Salmonellaon alfalfa seeds destined for sprout production. Int J Food Microbiol. 1997, 34: 329-333. 10.1016/S0168-1605(96)01202-0.View ArticlePubMedGoogle Scholar
- Holliday SL, Scouten AJ, Beuchat LR: Efficacy of chemical treatments in eliminating Salmonellaand Escherichia coliO157:H7 on scarified and polished alfalfa seeds. J Food Prot. 2001, 64: 1489-1495.PubMedGoogle Scholar
- Jaquette CB, Beuchat LR, Mahon BE: Efficacy of chlorine and heat treatment in killing Salmonella stanleyinoculated onto alfalfa seeds and growth and survival of the pathogen during sprouting and storage. Appl Environ Microbiol. 1996, 62: 2212-2215.PubMedPubMed CentralGoogle Scholar
- Scouten AJ, Beuchat LR: Combined effects of chemical, heat and ultrasound treatments to kill Salmonellaand Escherichia coliO157:H7 on alfalfa seeds. J Appl Microbiol. 2002, 92: 668-674. 10.1046/j.1365-2672.2002.01571.x.View ArticlePubMedGoogle Scholar
- Taormina PJ, Beuchat LR: Behavior of enterohemorrhagic Escherichia coliO157:H7 on alfalfa sprouts during the sprouting process as influenced by treatments with various chemicals. J Food Prot. 1999, 62: 850-856.PubMedGoogle Scholar
- Cuero RG, Smith JE, Lacey J: The influence of gamma irradiation and sodium hypochlorite sterilization on maize seed microflora and germination. Food Microbiol. 1985, 3: 107-113. 10.1016/S0740-0020(86)80034-X.View ArticleGoogle Scholar
- Sauer DB, Burroughs R: Disinfection of seed surfaces with sodium hypochlorite. Phytopathology. 1986, 76: 745-749.View ArticleGoogle Scholar
- Schultz T, Gabrielson RL: Control of Xanthomonas campestrispv. campestrisin crucifer seed with slurry treatments of calcium hypochlorite. Plant Dis. 1986, 70: 1027-1030.View ArticleGoogle Scholar
- FDA (Food and Drug Administration Center for Food Safety and Applied Nutrition): Guidance for Industry, Sampling And Microbial Testing Of Spent Irrigation Water During Sprout Production. Federal Register. 1999, 64 (207): 57893-57902.Google Scholar
- Mead PS, Slutsker L, Dietz V, McCaig LF, Bresee JS, Shapiro C, Griffin PM, Tauxe RV: Food-related illness and death in the United States. Emer Infect Dis. 1999, 5: 607-625.View ArticleGoogle Scholar
- Michino H, Araki K, Minami S, Takaya S, Sakai N, Miyazaki M, Ono A, Yanagawa H: Massive outbreak of Escherichia coli0157:H7 infection in schoolchildren in Sakai City, Japan, associated with consumption of white radish sprouts. Am J Epidemiol. 1999, 150: 787-796.View ArticlePubMedGoogle Scholar
- Taormina PJ, Beuchat LR, Slutsker L: Infections associated with eating seed sprouts: An international concern. Emerg Infect Dis. 1999, 5: 626-634.View ArticlePubMedPubMed CentralGoogle Scholar
- Mohie-Boetani JC, Farrar JA, Werner SB, Minassian D, Bryant R, Abbott S, Slutsker L, Vugia DJ: Escherichia coli0157 and Salmonellainfections associated with sprouts in California, 1996–1998. Ann Intern Med. 2001, 135: 239-247.View ArticleGoogle Scholar
- Hulebak KL, Schlosser W: Hazard analysis and critical control point (HACCP) history and conceptual overview. Risk Anal. 2002, 22: 547-552. 10.1111/0272-4332.00038.View ArticlePubMedGoogle Scholar
- FDA (U.S Food and Drug Administration Center for Food Safety and Applied Nutrition): CFSAN 2000 Program Priorities Report Card. December 5, 2000, [http://vm.cfsan.fda.gov/~dms/cfsand00.html]
- Wallace HA, Hammack TS: Chapter 5. Bacteriological Analytical Manual, Revision A. Edited by: Wallace HA, Hammack TS. 1998, 8Google Scholar
- Gilbert RO: Statistical Methods for Environmental Pollution Monitoring. 1987, New York, NY Van Nostrand ReinholdGoogle Scholar
- Feldsine PT, Falbo-Nelson MT, Brunelle SL, Forgey RL: Visual immunoprecipitate assay (VIP) for detection of enterohemorrhagic Escherichia coli(EHEC) O157:H7 in selected foods: collaborative study. J AOAC Int. 1997, 80: 517-529.PubMedGoogle Scholar
- AOAC International: AOAC Official Method 996.10. Enterohemorrhagic Escherichia coli(EHEC) O157:H7 Detection in Selected Foods. 2000, The Association of Official Analytical Chemists, Washington DC, 3-Google Scholar
- Neogen Technical Product Information. [http://www.neogen.com/pdf/FS_CatalogPages/RevealSalmonella.pdf]
- Feldsine PT, Mui LA, Forgey RL, Kerr DE: Equivalence of Visual Immunoprecipitate Assay (VIP) for Salmonellafor the detection of motile and nonmotile Salmonellain all foods to AOAC culture method: collaborative study. J AOAC Int. 2000, 83: 888-902.PubMedGoogle Scholar
- AOAC International: AOAC Official Method 996.08. Salmonella in Foods. The Association of Official Analytical Chemists, Washington, DC. 2000, 3 pp-Google Scholar
- Feldsine PT, Mui LA, Forgey RL, Kerr DE: Equivalence of assurance Gold Enzyme Immunoassay for visual or instrumental detection of motile and nonmotile Salmonella in all foods to AOAC culture method: collaborative study. J AOAC Int. 2000, 83: 871-887.PubMedGoogle Scholar
- AOAC International: AOAC Official Method 989.14. Salmonellain Foods. The Association of Official Analytical Chemists, Washington, DC. 2002, 4 pp-Google Scholar
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